MABN1802

Anti-pan-PMCA Antibody, clone 5F10

clone 5F10, from mouse

• Sinonimo/i: Plasma membrane calcium-transporting ATPase, Plasma membrane calcium pump
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 5F10, monoclonal
• Reattività contro le specie: chicken, rat, human
• Confezionamento: antibody small pack of 25 μg
• tecniche: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001001323.1; NP_001001331; NP_001001396.1; NP_068768.2
• N° accesso UniProt: P20020; P23634; Q01814; Q16720
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ATP2B1(490) , ATP2B3(492) , ATP2B4(493)

Descrizione

Descrizione generale

Plasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human. PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCAs serve as the main off switch for calcium signals in all types of cells. Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties. PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization. The localization of different PMCAs is regulated in various complex, cell type-specific ways. PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung. PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons. PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms. (Ref.: Antalffy, G et al. (2013). Biochim. Biophys. Acta 1833(12); 2561-2572; Strehler, EE (2015). Biochem. Biophys. Commun. 460 (1); 26-33)

Specificità

Clone 5F10 detects all isoforms of Plasma membrane calcium-transporting ATPase (PMCA) in human, mouse, and rat.

Immunogeno

Purified human erythrocyte calcium ATPase.

Applicazioni

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected pan-PMCA in human pancreas and human kidney tissue.

Immunocytochemistry Analysis: A representative lot detected pan-PMCA in Immunocytochemistry applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated pan-PMCA in Immunoprecipitation applications (DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Western Blotting Analysis: A representative lot detected pan-PMCA in Western Blotting applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

ELISA Analysis: A representative lot detected pan-PMCA in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

Research Category
Neuroscience

Qualità

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected pan-PMCA in human cerebral cortex tissue sections.

Descrizione del bersaglio

134 to 139 kDa calculated.

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash