MABD167

Anti-GATA1 Antibody, clone 4F5

ascites fluid, clone 4F5, from mouse

• Sinonimo/i: Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: 4F5, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2b
• N° accesso UniProt: P15976
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GATA1(2623)

Descrizione

Descrizione generale

GATA-1 (GATA1), or alternatively Gata-binding factor 1, GF-1, NF-E1 DNA binding protein, and Erythroid transcription factor (EryF1) encoded by the human gene named GATA1/ERYF1/GF1 is transcriptional activator central for erythroid development. GATA1 also plays critical roles in controlling gene expression in other system as well. Research has shown that GATA1 contributes to the level of alpha synuclein gene expression in Parkinson’s disease and over expression of GATA1 in cortical neurons leads to a decrease in the expression of Rab4b and reduces dendrite branching and maturation, and GATA1 expression is elevated in brains with depressive disorder suggesting that GATA1 may be new target for anti-depressant therapies. EMD-Millipore’s Anti-GATA1 mouse monoclonal antibody has been tested in western blot on K562 cell lysates and paraffin embedded immunohistochemistry on pancreatic cancer tissues and in fluorescent immunocytochemistry on K562 cells in culture.

Immunogeno

Purified recombinant fragment of human GATA1 expressed in E. Coli.

Applicazioni

Anti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in K562 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in pancreatic cancer tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Qualità

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GATA1 in K562 cell lysate.

Descrizione del bersaglio

~45 kDa observed. Uncharacterized bands may appear in some lysate(s).

Linkage

Replaces: MABE470

Stato fisico

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
K562 cell lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable