MAB312
Anti-A2B5 Antibody, clone A2B5-105
clone A2B5-105, Chemicon®, from mouse
Sinonimo/i:
Neuron Cell Surface Antigen
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
A2B5-105, monoclonal
Reattività contro le specie (prevista in base all’omologia)
mammals
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
Isotipo
IgM
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Specificità
Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
Immunogeno
Embryonic chicken retinal cells
Applicazioni
Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.
Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Stato fisico
Format: Purified
Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.
Stoccaggio e stabilità
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Type II astrocytes, human neural progenitors
Type II astrocytes, human neural progenitors
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Articoli
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
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