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MAB3060

Sigma-Aldrich

Anti-Paxillin Antibody, clone 349

clone 349, Chemicon®, from mouse

Sinonimo/i:

Anti-AW108311, Anti-AW123232, Anti-Pax

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100 μG
CHF 616.00

CHF 616.00


Spedizione prevista il17 maggio 2025


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Cambia visualizzazione
100 μG
CHF 616.00

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CHF 616.00


Spedizione prevista il17 maggio 2025


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

349, monoclonal

Reattività contro le specie

rat, human, mouse, canine, chicken

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PXN(5829)
rat ... Pxn(360820)

Specificità

Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.

Immunogeno

Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.

Applicazioni

Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Western blot: 1:1,000-1:10,000

ELISA

Immunohistochemistry 1:900 in Placenta

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-581

Stato fisico

Format: Purified
Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Risultati analitici

Control
POSITIVE CONTROL:

For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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M Ishino et al.
FEBS letters, 474(2-3), 179-183 (2000-06-06)
Hic-5 is a CAKbeta-binding protein localized at focal adhesions. Here we show that overexpression of CAKbeta or Fyn, but not FAK, enhanced the tyrosine phosphorylation of coexpressed Hic-5 in COS-7 cells. These phosphorylations were further augmented by stimulating cells with
Role of zyxin in differential cell spreading and proliferation of melanoma cells and melanocytes.
van der Gaag, EJ; Leccia, MT; Dekker, SK; Jalbert, NL; Amodeo, DM; Byers, HR
The Journal of Investigative Dermatology null
Mariam Mansour et al.
Journal of cell science, 129(2), 277-289 (2015-12-09)
Eph receptors and their corresponding membrane-bound ephrin ligands regulate cell positioning and establish tissue patterns during embryonic and oncogenic development. Emerging evidence suggests that assembly of polymeric Eph signalling clusters relies on cytoskeletal reorganisation and underlies regulation by protein tyrosine
Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals.
Stylianou, P; Skourides, PA
Mechanisms of Development null

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