CP28
Anti-Fibroblast Antigen Mouse mAb (AS02)
liquid, clone AS02, Calbiochem®
Sinonimo/i:
Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
AS02, monoclonal
Forma fisica
liquid
contiene
≤0.1% sodium azide as preservative
Reattività contro le specie
human, monkey
Non deve reagire con
rat, mouse
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Isotipo
IgG1
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... FGF22(27006)
Descrizione generale
Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
Immunogeno
Human
human fibroblasts
Applicazioni
Flow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Confezionamento
Please refer to vial label for lot-specific concentration.
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
In 50 mM sodium phosphate buffer, 6% BSA.
Risultati analitici
Positive Control
Any fibroblast cell line or skin tissue
Any fibroblast cell line or skin tissue
Altre note
Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Note legali
Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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