This product does not indicate inclusion of dimedone or any labeling reagent. The antibody detects the sulfenic acid-dimedone adduct formed when sulfenic acid is trapped by dimedone. Dimedone must be purchased separately. Please see the links below to review dimedone that may be of interest.
https://www.sigmaaldrich.com/search/dimedon?focus=products&page=1&perpage=30&sort=relevance&term=dimedon&type=product
ABS30
Anti-Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig) Antibody
serum, from rabbit
Sinonimo/i:
Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig)
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 μL | Per conoscere la disponibilità, visualizza il carrello | CHF 453.00 |
Informazioni su questo articolo
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
WB
Citations:
9
Servizio Tecnico
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Permettici di aiutartibiological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
species reactivity (predicted by homology)
all
technique(s)
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.
Pan antibody smear is expected.
Immunogen
Linear peptide corresponding to sulfenic acid modified proteins.
Application
Anti-Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig) Antibody detects level of Sulfenic Acid Modified Cysteine & has been published & validated for use in WB.
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Seo, YH, et al. (2009). PNAS. 106(38): 16163-16168.)
Biochem/physiol Actions
This anitbody recognizes sulfenic acid modified proteins. Pan modification against all species.
Analysis Note
Evaluated by Western Blot in rat ventricular myocyte lysate.
Western Blot Analysis: 01:1,000 dilution of this antibody detected sulfenic acid modified proteins on 10 µg of rat ventricular myocyte lysate.
Western Blot Analysis: 01:1,000 dilution of this antibody detected sulfenic acid modified proteins on 10 µg of rat ventricular myocyte lysate.
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Questo articolo | |||
|---|---|---|---|
| description serum, from rabbit | description clone C4, from human(Recombinant) | description serum, from rabbit | description from non-animal source, BioReagent, suitable for cell culture, ≥98% |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| biological source rabbit | biological source human (Recombinant) | biological source rabbit | biological source non-animal source |
| antibody form serum | antibody form purified immunoglobulin | antibody form serum | antibody form - |
| clone polyclonal | clone C4, monoclonal | clone polyclonal | clone - |
| technique(s) western blot: suitable | technique(s) western blot: suitable, ELISA: suitable | technique(s) western blot: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
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Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 1
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This antibody (ABS30) does not directly detect -SOH; rather, it recognizes the structure formed when dimedone binds to sulfenic acid? When I purchase the antibody, does it come with dimedone? Or do I need to purchase it separately?
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