AB175

Anticorpo FLAG

serum, Chemicon^®

• Sinonimo/i: Anticorpo anti-recettore GABA, Anticorpo per recettore GABA
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat
• Application: ELISA, IHC
• Citations: 64

Proprietà

• biological source: guinea pig
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable (paraffin)
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Gabra1(29705)

Descrizione

General description

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. In this reaction pyridoxal phosphate serves as a co-factor. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. − This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. − GABAB receptors are coupled indirectly to K+ channels. When activated, GABAB receptors reduce Calcium ion conductance and inhibit cAMP production via intracellular mechanisms mediated by G-proteins. GABAB receptors are known to mediate both postsynaptic and presynaptic inhibition.

Immunogen

BSA-conjugated GABA-Glutaraldehyde

Application

Analisi immunoistochimica: un lotto rappresentativo alla diluizione 1:500 ha permesso di rivelare NeuN in cellule di corteccia di ratto E18.

Saggio immunoistochimico:



Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

Nota: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

Categoria di ricerca
Neuroscienze

Sottocategoria di ricerca
Neurotrasmettitori & recettori

This Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.

Biochem/physiol Actions

GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.

Recognizes GABA. μ μ

Physical form

Siero HI (inattivato col calore)

Siero di anticorpo policlonale di cavia contenente azoturo di sodio allo 0,05%.

Preparation Note

Stabile per 1 anno dalla data di ricezione a -20°C.
Raccomandazioni per la manipolazione: una volta ricevuto il prodotto, prima di rimuovere il tappo, centrifugare il contenitore e mescolare delicatamente la soluzione. Suddividere in aliquote in provette da microcentrifuga e conservare a -20°C. Evitare ripetuti cicli di congelamento e scongelamento che potrebbero danneggiare le IgG e compromettere le prestazioni del prodotto.

Analysis Note

Controllo
Tessuto oculare di ratto

Saggiata mediante immunoistochimica su tessuto oculare di ratto.

Saggio immunoistochimico: una diluizione 1:500 di questo anticorpo ha rilevato la doublecortina nel lisato di tessuto cerebrale di ratto.

Other Notes

Concentrazione: variabile.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Salvo diversa indicazione nel nostro catalogo o in altra documentazione fornita dall′azienda insieme al prodotto, i nostri prodotti sono destinati esclusivamente a scopi di ricerca e non devono essere utilizzati per altre finalità, inclusi a titolo esemplificativo ma non esaustivo, fini commerciali non autorizzati, applicazioni diagnostiche in vitro, usi terapeutici ex vivo o in vivo o qualsiasi altro tipo di assunzione o applicazione rivolta agli esseri umani o agli animali.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable