70920
Rosetta(DE3)pLacI Competent Cells - Novagen
Escherichia coli, rod shaped
About This Item
Prodotti consigliati
Nome del prodotto
Rosetta(DE3)pLacI Competent Cells - Novagen, Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
Origine biologica
Escherichia coli
Livello qualitativo
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
Modalità di accrescimento
adherent or suspension
Morfologia
rod shaped
tecniche
microbiological culture: suitable
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Descrizione generale
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.
.Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Componenti
•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)pLacI Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Attenzione
Note legali
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