When autoclaved or filter sterilized, the prepared solution may be stored tightly sealed at room temperature for up to 2 years.
M6030
M9, Minimal Salts, 5X
powder, minimal microbial growth medium
Sinonimo/i:
M9 media
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1 KG
CHF 295.00
CHF 295.00
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Cambia visualizzazione
1 KG
CHF 295.00
About This Item
Codice UNSPSC:
12352302
NACRES:
NA.85
CHF 295.00
Check Cart for Availability
Prodotti consigliati
Grado
Molecular Biology
for molecular biology
Livello qualitativo
Sterilità
non-sterile
Stato
powder
Composizione
KH2PO4, 15 g/L
NaCl, 2.5 g/L
Na2HPO4, 33.9 g/L
NH4Cl, 5 g/L
applicazioni
food and beverages
Temperatura di conservazione
room temp
Compatibilità
nonselective for Escherichia coli
nonselective for coliforms
Descrizione generale
M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.
Applicazioni
M9, Minimal Salts, 5X has been used:
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
Caratteristiche e vantaggi
M9 powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
Nota sulla preparazione
1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
Ricostituzione
Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Protocolli
General protocols for growth of competent cells in microbial medium.
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Where does the 5x concentrate need to be stored? At room temperature or at 4°C? And how long would the shelf life be?
1 answer-
Helpful?
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What is the preparation ratio for the culture medium?
1 answer-
The preparation instructions are listed on the product detail page in the DESCRIPTION section under 'Preparation'.
Please navigate to the link below:
https://www.sigmaaldrich.com/product/sigma/m60301. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5X concentrate can be stored and diluted as needed to prepare 5 L of 1X M9 minimal salts.For M9 minimal medium:
1. Aseptically dilute 200 mL of M9 minimal salts, 5X concentrate with 800 mL of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 mL of sterile 1 M glucose and 2 mL of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 mL of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.Helpful?
-
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