676602

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

lyophilized, clone 16C2, Calbiochem^®

• Sinonimo/i: Anti-Vasodilator-stimulated Phosphoprotein, Activated
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 16C2, monoclonal
• Forma fisica: lyophilized
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: mouse, human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG1
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer239/pSer239)

Descrizione

Descrizione generale

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

Immunogeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH

Applicazioni

ELISA (0.05 µg/ml)

Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 2X PBS, PEG, sucrose.

Ricostituzione

Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Risultati analitici

Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets

Altre note

Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.

Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable