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Sigma-Aldrich

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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About This Item

Codice UNSPSC:
12352200

Livello qualitativo

Forma fisica

liquid

Attività specifica

≥20,000 units/mL

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Attività estranea

Endonuclease and RNase, none detected

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Azioni biochim/fisiol

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Stato fisico

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Ricostituzione

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Altre note

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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