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Sigma-Aldrich

Cdc42 Activation Assay Kit

Non-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Livello qualitativo

Produttore/marchio commerciale

Upstate®

tecniche

activity assay: suitable
affinity binding assay: suitable

N° accesso UniProt

Metodo di rivelazione

chemiluminescent

Condizioni di spedizione

wet ice

Informazioni sul gene

human ... CDC42(998)

Confezionamento

Kit capacity: 30 assays

Componenti

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Anti-cdc42

Qualità

Routinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.

Linkage

Replaces: SGT430

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

CorrosionEnvironment

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Codice della classe di stoccaggio

10 - Combustible liquids


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Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
Zhi-Jie Cheng, Raman Deep Singh, Deepak K Sharma, Eileen L Holicky, Kentaro Hanada et al.
Molecular Biology of the Cell null
Isao Usui et al.
The Journal of biological chemistry, 278(16), 13765-13774 (2003-02-05)
We investigated the role of cdc42, a Rho GTPase family member, in insulin-induced glucose transport in 3T3-L1 adipocytes. Microinjection of anti-cdc42 antibody or cdc42 siRNA led to decreased insulin-induced and constitutively active G(q) (CA-G(q); Q209L)-induced GLUT4 translocation. Adenovirus-mediated expression of
Mark C Wilkes et al.
Developmental cell, 16(3), 433-444 (2009-03-18)
Transforming growth factor beta (TGF-beta) family ligands are pleotropic proteins with diverse cell-type-specific effects on growth and differentiation. For example, PAK2 activation is critical for the proliferative/profibrotic action of TGF-beta on mesenchymal cells, and yet it is not responsive to
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
S J Taylor et al.
Current biology : CB, 6(12), 1621-1627 (1996-12-01)
Ras proteins play an essential role in the transduction of signals from a wide range of cell-surface receptors to the nucleus. These signals may promote cellular proliferation or differentiation, depending on the cell background. It is well established that Ras

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