16-318

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

clone SKB1, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: SKB1, monoclonal
• Reattività contro le specie: mouse, rat, human
• tecniche: immunoprecipitation (IP): suitable
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Akt1(11651)

Descrizione

Descrizione generale

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Specificità

Recognizes Akt1/PKBα.

Immunogeno

GST-fusion protein corresponding to residues 1-149 of human Akt1

Applicazioni

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Qualità

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

Descrizione del bersaglio

~ 60 kDa observed

Stato fisico

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Format: Purified

Protein G Chromatography

Stoccaggio e stabilità

Stable for 1 year at 2-8ºC from date of receipt.

Risultati analitici

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2