05-414
Anti-CrkL Antibody, clone 5-6
clone 5-6, Upstate®, from mouse
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
5-6, monoclonal
Reattività contro le specie
mouse, rat, human
Produttore/marchio commerciale
Upstate®
tecniche
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2aκ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... CRKL(1399)
Descrizione generale
CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.
The antibody does not cross-react with CRKI or CRKII.
Specificità
Other species have not been tested.
the C-terminal SH3 of Human CRKL
Immunogeno
Epitope: Full-length
Full length GST fusion protein corresponding to Human CRKL
Applicazioni
Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Qualità
Routinely evaluated by Western Blot on K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Descrizione del bersaglio
39kDa
Stato fisico
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Stoccaggio e stabilità
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Risultati analitici
Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway
Sattler, M, et al
Oncogene, 12, 839-846 (1996)
IL-4 regulates the expression of CD209 and subsequent uptake of Mycobacterium leprae by Schwann cells in human leprosy.
Teles RM, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, Modlin RL
Infection and Immunity null
Atsushi Oda et al.
The Journal of biological chemistry, 278(8), 6456-6460 (2003-01-11)
Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP)
Eike R Hrincius et al.
Cellular microbiology, 12(6), 831-843 (2010-01-22)
The non-structural protein 1 (A/NS1) of influenza A viruses (IAV) harbours several src-homology domain (SH) binding motifs that are required for interaction with cellular proteins. The SH3 binding motif at aa212-217 [PPLPPK] of A/NS1 was shown to be essential for
Cellular interactions of CRKL, and SH2-SH3 adaptor protein
ten Hoeve, J, et al
Cancer Research, 54, 2563-2567 (1994)
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