Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

Visualizza tutta la documentazione

909599

Sigma-Aldrich

DHSO crosslinker

Sinonimo/i:

3,3′-Sulfinyldi(propanehydrazide), Dihydrazide sulfoxide, Mass spectrometry-cleavable crosslinker for studying protein-protein interations

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

100 MG
CHF 299.00

CHF 299.00

Check Cart for Availability
Richiedi un ordine bulk
Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
100 MG
CHF 299.00

About This Item

Formula empirica (notazione di Hill):
C6H14N4O3S
Numero CAS:
1971857-82-5
Peso molecolare:
222.27
Numero MDL:
MFCD32851664
Codice UNSPSC:
12161502
NACRES:
NA.22

CHF 299.00

Check Cart for Availability
Richiedi un ordine bulk

Stato

powder

Temperatura di conservazione

2-8°C

Stringa SMILE

[S](=O)(CCC(=O)NN)CCC(=O)NN

InChI

XTCXQISMAWBOOT-UHFFFAOYSA-N

Applicazioni

DHSO Crosslinker is a homobifunctional, acidic residue-targeting, sulfoxide-containing crosslinker for analysis of protein-protein interactions (PPIs) through crosslinking mass spectrometry (XL-MS). DHSO possesses two hydrazide-reactive groups for targeting aspartic acid (Asp) and glutamic acid (Glu) and two symmetrical C-S cleavable bonds adjacent to the central sulfoxide. While former amine-reactive reagents react with lysine (Lys) residues in proximity to the PPI, targeting acidic residues provides further opportunity to characterize protein interaction surfaces at which Asp and Glu are often prevalent. Additionally, the 12.4 Å spacer yields tagged peptides post-cleavage for unambiguous identification by collision-induced dissociation in tandem MS. DHSO Crosslinker provides complementary data to Lys-targeting reagents and will find wide utility in the elucidation of PPIs, study of proteins that function as complexes, quantification of structural dynamics, and quest for targeting "undruggable" protein targets.

Altre note

Technology Spotlight: Cross-Linkers for Elucidation of Protein-Protein Interactions

Developing an Acidic Residue Reactive and Sulfoxide-Containing MS-Cleavable Homobifunctional Cross-Linker for Probing Protein–Protein Interactions

Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein–Protein Interactions

Structural dynamics of the human COP9 signalosome revealed by cross-linking mass spectrometry and integrative modeling

Note legali

Subject to US Patent Application #15/613,065 of the Regents of the University of California

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

909610

BMSO crosslinker, ≥95%

909629

Azide-A-DSBSO crosslinker, ≥95%

909602

DSSO crosslinker, ≥95%

909661

d0-DMDSSO crosslinker, ≥95%

909688

Alkyne-A-DSBSO crosslinker, ≥95%

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
MKCW0183
MKCT9690
MKCQ4172
MKCM0988

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Craig B Gutierrez et al.
Analytical chemistry, 88(16), 8315-8322 (2016-07-16)
Cross-linking mass spectrometry (XL-MS) has become a powerful strategy for defining protein-protein interactions and elucidating architectures of large protein complexes. However, one of the inherent challenges in MS analysis of cross-linked peptides is their unambiguous identification. To facilitate this process
Craig B Gutierrez et al.
Analytical chemistry, 90(12), 7600-7607 (2018-05-25)
Cross-linking mass spectrometry (XL-MS) has become an emerging technology for defining protein-protein interactions (PPIs) and elucidating architectures of large protein complexes. Up to now, the most widely used cross-linking reagents target lysines. Although such reagents have been successfully applied to
Craig Gutierrez et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(8), 4088-4098 (2020-02-09)
The COP9 signalosome (CSN) is an evolutionarily conserved eight-subunit (CSN1-8) protein complex that controls protein ubiquitination by deneddylating Cullin-RING E3 ligases (CRLs). The activation and function of CSN hinges on its structural dynamics, which has been challenging to decipher by

Articoli

MS-Cleavable Cross-Linkers

Sulfoxide-containing MS-cleavable cross-linkers capture protein-protein interactions in native cellular environments, aiding PPI identification.

Crosslinkers Selection Guide

Choosing the right crosslinker for your biochemical or bioconjugation research can be challenging. Our selection guide can help you find the perfect match for your applications.

Guida alla scelta dei reticolanti (agenti di cross-linking)

La scelta del reticolante opportuno per studi di biochimica o di bioconiugazione può essere impegnativa. La nostra guida alla scelta può aiutarvi a trovare l'abbinamento perfetto per le vostre applicazioni.

Questions

Reviews

No rating value

Active Filters

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.