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E7750
N-(3-dimetilaminopropil)-N′-etilcarbodiimide
commercial grade, powder
Sinonimo/i:
N-etil-N′-(3-dimetilaminopropil)carbodiimide, EDAC, EDC, WSC
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| A voi/SKU | Disponibilità | Prezzo |
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Informazioni su questo articolo
Quality Segment
form
powder
technique(s)
Northern blotting: suitable, bioconjugation: suitable
color
white to off-white
mp
110-115 °C (lit.)
solubility
H2O: ≤100 mg/mL
storage temp.
−20°C
SMILES string
Cl.CCN=C=NCCCN(C)C
InChI
1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key
FPQQSJJWHUJYPU-UHFFFAOYSA-N
General description
La versatilità dell′EDC HCl si rivela ulteriormente nella sua capacità di modificare gli acidi nucleici, rendendo possibile la marcatura di DNA ed RNA a livello dei rispettivi gruppi 5′ fosfato. Tale marcatura migliora la visualizzazione, il tracciamento e l′analisi di molecole così importanti, contribuendo in modo significativo all′avanzamento della ricerca sugli acidi nucleici. Per di più, l′EDC HCl può essere impiegato come ponte biomolecolare essenziale, agendo da crosslinker e attivando la coniugazione tra esteri N-idrossisuccinimmidici (esteri NHS) di biomolecole e biomolecole reattive a livello di gruppi amminici. Questa tecnica si rivela particolarmente utile nella coniugazione di proteine mirata alla creazione di una molecola ibrida con nuove proprietà e nuove funzioni. Il meccanismo soggiacente prevede che l′EDC HCl reagisca con il gruppo carbossilico di una prima proteina, con formazione di un intermedio instabile propenso ad accoppiarsi con il gruppo amminico libero di una seconda proteina. Il delicato equilibrio di questa reazione richiede l′ottimizzazione delle condizioni di reazione perché si giunga ad una coniugazione efficace. Il coinvolgimento dell′N-idrossisuccinimmide (NHS) migliora i risultati ottenibili con l′intervento del solo EDC HCl, dando corso ad un processo di coniugazione in due passaggi che offre una maggiore flessibilità e un maggior controllo sulla reazione, specialmente nel caso di biomolecole complesse.
Application
- Il cloridrato di N-(3-dimetilamminopropil)-N′-etilcarbodiimmide è stato usato per la generazione di bioconiugati trasferrina-nanodiamanti fluorescenti (FND).
- È stato usato per il crosslinking della polietilenimmina su particelle d′oro.[1]
- È stato usato come agente di legame della carbodiimmide per il rivestimento di sferette di polistirene carbossilato con albumina di siero bovino (BSA) biotinilata.
Biochem/physiol Actions
Features and Benefits
Other Notes
1 of 1
Questo articolo | |||
|---|---|---|---|
| form powder | form crystalline | form solid | form powder |
| technique(s) Northern blotting: suitable, bioconjugation: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C (−15°C to −25°C) | storage temp. −20°C |
| mp 110-115 °C (lit.) | mp 110-115 °C (lit.) | mp 110-115 °C | mp 110-115 °C (lit.) |
| color white to off-white | color white to off-white | color - | color - |
| solubility H2O: ≤100 mg/mL | solubility H2O: ≤100 mg/mL | solubility - | solubility H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow |
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Danger
target_organs
Stomach,large intestine,lymph node
Classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What impurities might be present in N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750?
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The two compounds most likely to appear in EDAC as trace impurities are 3-dimethylaminopropylamine and N-(3-dimethylaminopropyl)-N'-ethylurea as a result of synthesis (intermediate) and degradation (moisture or coupling reaction), respectively.
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Product No. E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, is listed as "commercial grade". What does that mean?
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It means that it is the grade of N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide (EDAC) hydrochloride that was commercially available at the time we purchased it. This product is not assayed for purity. If you need a high purity EDAC, consider Product No. 03450.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750, be used as a peptide coupling reagent in alcoholic solvents? If so, do you have a reference?
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Yes. Nosaki, S., Effects of amounts of additives on peptide coupling mediated by a water-soluble carbodiimide in alcohols. J. Peptide Res., 54(2), 162-167 (1999). This paper describes using the E7750 along with 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HOOBt) and 1-hydroxy-7-azabenzotriazole (HOAt) for enhancement of peptide coupling.
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I understand that E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, can be used in peptide synthesis. Do you have a brochure on some other reagents that can be used for peptide synthesis?
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Yes.
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