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Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.

Cell (2023-05-13)
Michael D Healy, Kerrie E McNally, Rebeka Butkovič, Molly Chilton, Kohji Kato, Joanna Sacharz, Calum McConville, Edmund R R Moody, Shrestha Shaw, Vicente J Planelles-Herrero, Sathish K N Yadav, Jennifer Ross, Ufuk Borucu, Catherine S Palmer, Kai-En Chen, Tristan I Croll, Ryan J Hall, Nikeisha J Caruana, Rajesh Ghai, Thi H D Nguyen, Kate J Heesom, Shinji Saitoh, Imre Berger, Christiane Schaffitzel, Tom A Williams, David A Stroud, Emmanuel Derivery, Brett M Collins, Peter J Cullen
RÉSUMÉ

The Commander complex is required for endosomal recycling of diverse transmembrane cargos and is mutated in Ritscher-Schinzel syndrome. It comprises two sub-assemblies: Retriever composed of VPS35L, VPS26C, and VPS29; and the CCC complex which contains twelve subunits: COMMD1-COMMD10 and the coiled-coil domain-containing (CCDC) proteins CCDC22 and CCDC93. Combining X-ray crystallography, electron cryomicroscopy, and in silico predictions, we have assembled a complete structural model of Commander. Retriever is distantly related to the endosomal Retromer complex but has unique features preventing the shared VPS29 subunit from interacting with Retromer-associated factors. The COMMD proteins form a distinctive hetero-decameric ring stabilized by extensive interactions with CCDC22 and CCDC93. These adopt a coiled-coil structure that connects the CCC and Retriever assemblies and recruits a 16th subunit, DENND10, to form the complete Commander complex. The structure allows mapping of disease-causing mutations and reveals the molecular features required for the function of this evolutionarily conserved trafficking machinery.

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