Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53313-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
53313-U
Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns
L × I.D. 10 cm × 3 mm, HPLC Column
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About This Item
Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Nom du produit
Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 3 mm
Matériaux
stainless steel column
Niveau de qualité
Agence
suitable for USP L1
Gamme de produits
Ascentis®
Caractéristiques
endcapped: no
Fabricant/nom de marque
Ascentis®
Conditionnement
1 ea of
Paramètres
≤100 °C temp. range
600 bar max. pressure (9000 psi)
Technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × D.I.
10 cm × 3 mm
Superficie
90 m2/g
Impuretés
<5 ppm metals
Matrice
Fused-Core particle platform
superficially porous particle
Groupe de la matrice active
C18 (octadecyl) phase
Taille des particules
2.7 μm
Dimension de pores
160 Å
Plage de pH de fonctionnement
1-9
Technique de séparation
reversed phase
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Description générale
Ascentis Express Peptide ES-C18 columns are specifically engineered to separate higher molecular weight compounds such as peptides and small proteins. These columns contain advanced Fused-Core particles that have larger pores (160 Å versus 90 Å in standard Ascentis Express), bonded with sterically-protected C18 ligands to provide extra stability (ES) at very low pH (< 1) and high temperatures (up to 100ºC). This greatly expands the application range for Ascentis Express columns.
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
Réf. du produit
Description
Tarif
Colonne de garde
Réf. du produit
Description
Tarif
Nécessaire, mais non fourni
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Articles
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
Chromatograms
application for HPLC-
What flow rate should I use with product 53313-U Ascentis® Express Peptide ES C18 HPLC columns?
1 answer-
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What is special about product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Product 53313-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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Are guard columns available for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Yes, we have guard columns available for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53537-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 3.0 mm .
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Can product 53313-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?
1 answer-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53313-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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What is the pressure rating for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
This product 53313-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)
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Can I use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 answer-
Yes. product 53313-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53313-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53313-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the carbon load for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53313-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is there anything special I need to do to my HPLC system to use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Nothing special is required to use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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