ZRB1204

Anti-p300 Antibody, clone 4E5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4E5, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 264.16 kDa; observed mol wt ~280 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: N-terminal half
• Numéro d'accès Protein ID: NP_001420.2
• Numéro d'accès UniProt: Q09472
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 4E5 a ZooMAb^® Rabbit recombinant monoclonal antibody that detects Histone acetyltransferase p300. It targets an epitope within the N-terminal half.

Immunogène

His-tagged recombinant fragment corresponding to 233 amino acids from the N-terminal half of human Histone acetyltransferase p300.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p300 in MCF-7 cells.

Tested applications

ELISA Analysis: 300 μg/mL from a representative lot detected p300 in an ELISA.

Affinity Binding Assay: A representative lot of this antibody bound p300 with a KD of 2 x 10-7 in an affinity binding assay.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected p300 in nuclear extracts from HeLa and NTERA2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p300, clone 4E5 ZooMAb^®, Cat. No. ZRB1204, is a recombinant Rabbit monoclonal antibody that detects p300 (Histone Acetyltransferase p300) and is tested for use Affinity Binding Assay, ELISA, Immunocytochemistry, and Western Blotting.

Description de la cible

Histone acetyltransferase p300 (UniProt: Q09472; also known as EC:2.3.1.48, p300 HAT, E1A-associated protein p300, Histone butyryltransferase p300, Histone crotonyltransferase p300, Protein 2-hydroxyisobutyryltransferase p300, Protein propionyltransferase p300) is encoded by the EP300 (also known as P300) gene (Gene ID: 2033) in human. p300 serves as a transcriptional co-activator protein that plays a central role in coordinating and integrating multiple signal-dependent transcriptional events, such as cell proliferation, differentiation, and apoptosis. It has histone acetyltransferase (HAT) activity that transfers an acetyl group to the -amino group of multiple lysine residues. p300 localizes to active chromatin and acetylates all four core histones in nucleosomes, providing an epigenetic tag for transcriptional activation. It also shown to mediate cAMP-gene regulation by binding specifically to phosphorylated CREB protein. It mediates acetylation of histone H3 at lysine 122, a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. It also mediates acetylation of histone H3 at lysine 27. It can also acetylase a number of non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Its acetylation of HDAC1 is shown to lead to inactivation and modulation of transcription. p300 contains one TAZ-type 1 (aa 331-417), one TAZ-type 2 (aa 1728-1809), and one ZZ-type (aa 1664-1707) zinc finger domains. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable