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Merck
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Principaux documents

Z380059

Sigma-Aldrich

Membrane d′étanchéité Breathe-Easy®

Breathe-Easy® sealing membrane
2 of 6 reviewers received a sample product or took part in a promotion

polyurethane membrane with acrylic adhesive pre-cut to fit standard multiwell plates.

Synonyme(s) :

breathable sealing membrane, cell culture, microplates, microwell plates, multi-well plates, multi-well sealing film, plate sealers, plate sealing film, sealing film, tissue culture

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100 EA
114.00 CHF

114.00 CHF

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100 EA
114.00 CHF

About This Item

Code UNSPSC :
41122410
Nomenclature NACRES :
NB.22

114.00 CHF

Check Cart for Availability

Matériaux

rectangular transparent membrane (polyurethane)

Stérilité

sterile

Caractéristiques

piercable

Conditionnement

pack of 100 ea

Fabricant/nom de marque

Diversified Biotech BEM-1

Catégories apparentées

Description générale

La membrane Breathe-Easy® est perméable à l'oxygène, au dioxyde de carbone et à la vapeur d'eau, ce qui permet de créer des récipients de microculture séparés et très uniformes sur une plaque multipuits.
Elle se présente sous la forme d'un sandwich : une feuille de papier inférieure (doublure) protège la couche adhésive et une feuille de support supérieure protège la surface externe de la membrane ultra-souple jusqu'à ce qu'elle soit positionnée et collée sur la plaque. La doublure garantit en outre la stérilité de la membrane. L'adhésif scelle chaque puits hermétiquement (à la différence des couvercles en plastique moulé), ce qui permet de réaliser une agitation sans contamination croisée ni infiltration microbienne. Stable entre −80 °C et +100 °C, cette membrane est compatible avec la plupart des produits caustiques et des solvants organiques.
Remarque : Il est recommandé d'utiliser le rouleau étaleur (réf. Z380067, vendu séparément) pour garantir une adhésion uniforme et faciliter le retrait de la feuille de papier supérieure.

Caractéristiques et avantages

Membrane Breathe-Easy® :
  • Stérile et non cytotoxique : stérilisée par rayonnement gamma (dépourvue de cytotoxines)
  • Prévient les contaminations : évite les contaminations inter-puits, les contaminations microbiennes et virales, l'évaporation (hors membranes respirantes) et le déversement accidentel des échantillons
  • Dimensionnée et conçue de manière optimale : rectangles de 6" × 3,25" qui s'adaptent sur toutes les microplaques ; deux côtés rainurés qui facilitent le positionnement et le retrait de la membrane
  • Thermostable : conçue pour résister à une large plage de températures
  • Perforable : facilite le prélèvement des échantillons avec une pointe de pipette

Informations légales

Breathe-Easy is a registered trademark of Diversified Biotech

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Victoria Hsiao et al.
ACS synthetic biology, 4(2), 150-161 (2014-05-23)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this
Teng Wang et al.
Nature communications, 11(1), 5589-5589 (2020-11-06)
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid's ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the
Adithi R Varadarajan et al.
NPJ biofilms and microbiomes, 6(1), 46-46 (2020-11-01)
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a
Nathan A Sallee et al.
SLAS discovery : advancing life sciences R & D, 25(9), 1047-1063 (2020-07-28)
The identification of novel peptide hormones by functional screening is challenging because posttranslational processing is frequently required to generate biologically active hormones from inactive precursors. We developed an approach for functional screening of novel potential hormones by expressing them in
Filipe Pinto et al.
Nature communications, 11(1), 1529-1529 (2020-04-07)
Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing
6 Reviews This action will navigate to reviews.

Questions

1–5 of 5 Questions  

  1. Is this product optically transparent? Can it be used for luminescence experiments?

    1 answer

    1. This product is distributed on behalf of Diversified Biotech. The films have not been tested for use in luminescence, but are UV transparent to 300 nm.

      Helpful?

  2. Hello! We use breath-easy membrane routinely in our lab. I need to test an effect of gas on bacterial growth. for that i want to know what is the permeability of membrane like what is the pore size?

    1 answer

    1. The material has no pores. The oxygen exchange rate is 553.5 cc per 100 square inches per 24 hours. The carbon dioxide exchange rate is 3781 cc per 100 square inches per 24 hours. The water vapor exchange rate is 0.7 kg per square meter per 24 hours.

      Helpful?

  3. Ces films sont-ils adaptés pour de la culture cellulaire? Remplacent-ils le couvercle ou doit-on mettre un film (stérile?) et le couvercle de la plaque dessus?

    1 answer

    1. Yes, these sealing films are sterile and suitable for use in cell culture. When in place, a plate lid is not needed. These films allow for the exchange of oxygen, carbon dioxide, and water vapors while preventing microbial/viral contamination and well-to-well cross contamination.

      Helpful?

  4. Is the Breath-easy sealing meant to be used with or without a lid? does it prevent contamination from leaking in without a lid?

    1 answer

    1. The adhesive seals are meant to be used without a lid. These items provide a secure seal for every well which prevents microbial/viral contamination and well-to-well cross-contamination.

      Helpful?

  5. Is it plate reader friendly for absorbance wavelength

    1 answer

    1. This product is distributed on behalf of Diversified Biotech. The original manufacturer states that these films are UV transparent to 300 nm.

      Helpful?

Reviews

2 of 6 reviewers received a sample product or took part in a promotion
1–3 of 6 Reviews  

Active Filters

    • Review 1
    • Votes 9
    1 out of 5 stars.

    transparent breath easy sealing membranes for microtiter readers

    We bought the Z380059, Breathe-Easy® sealing membrane to measure the growth curve of aerobic bacteria in microtiter plate. From the description of the product it sounds like this is the application they are sold for and in the picture from the producer the membranes look totally transparent. But when we received the product the membranes were rather opaque and could not be used for cell density reading. We contacted the supplier and received this answer: "The manufacturer of Z380059, Breathe-Easy® sealing membrane, has advised that these films are not transparent. Unfortunately we do not have any sealing films that are both breathable and optically clear."

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. SF
    • Review 1
    • Votes 2
    5 out of 5 stars.

    superior performance at -80C

    i love this product for its reliable performance, especially at -80 degree C

    Helpful?

    • Review 1
    • Votes 0
    1 out of 5 stars.

    A lot of air bubbles and media leaking out on 384 well plates

    We used the Breath-Easy with a plate lid because we were afraid of contamination. When we came on the next day it was a big mess of media leaking out, air bubbles and definitely cross contamination between the wells. Is the Breath-easy sealing meant to be used without a lid? without contamination risk?

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    Helpful?

    1. Response from MilliporeSigma:

      Breathe-Easy® sealing membranes are intended to prevent cross contamination and do not require use of a lid. When applying the seal, take care to press the adhesive to the cell openings to make sure there is a tight seal on each well.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  4. 3 Ratings-Only Reviews

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