XNAT2R
Extract-N-Amp™ Tissue PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonyme(s) :
Tissue direct PCR kit
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About This Item
Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55
Produits recommandés
Utilisation
sufficient for 1000 amplifications
sufficient for 1000 extractions
sufficient for 1000 reactions
Caractéristiques
dNTPs included
hotstart
Technique(s)
PCR: suitable
Couleur
colorless
Conditions d'expédition
wet ice
Température de stockage
−20°C
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Catégories apparentées
Description générale
The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by direct PCR (polymerase chain reaction) . A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.
Application
Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and polymerase chain reaction (PCR).
Caractéristiques et avantages
- Fast – rapid extraction of genomic DNA for PCR in 15 minutes
- Convenient – no long enzymatic digestions or column purification required
- Practical – perfect for quick genomic DNA isolation for genotyping
- Flexible – protocols available for mouse-tails, hair, animal tissue, saliva, and buccal swabs
- Specific – Hot start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 oC for at least 6 months
Composants
Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.
Principe
The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.
Autres remarques
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
This kit includes a clear solution of PCR readymix. To order the REDExtract-N-Amp Tissue PCR Kits with the inert tracking and loading dye in the PCR readymix, please order XNAT-1000RXN"
This kit includes a clear solution of PCR readymix. To order the REDExtract-N-Amp Tissue PCR Kits with the inert tracking and loading dye in the PCR readymix, please order XNAT-1000RXN"
Informations légales
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Articles
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Protocoles
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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