T5050

Transforming Growth Factor-β1 from porcine platelets

≥97% (SDS-PAGE), recombinant, powder, suitable for cell culture

• Synonyme(s) : TGF-β1
• Numéro MDL: MFCD00166089
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Transforming Growth Factor-β1 from porcine platelets, TGF-β1, powder, suitable for cell culture
• Source biologique: Porcine (Platelets)
• Niveau de qualité: 100
• Pureté: ≥97% (SDS-PAGE)
• Forme: powder
• Puissance: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Poids mol.: protein 25 kDa
• Conditionnement: pkg of 1 μg; pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P07200
• Température de stockage: −20°C
• Informations sur le gène: pig ... TGFB1(397078)

Description

Description générale

The TGFB (transforming growth factor-β) gene is mapped to pig (Sus scrofa) chromosome between 6q11-q21. In mammals, TGFβ is present in three isoforms (TGFβ1, TGFβ2 and TGFβ3), of which TGFβ1 is predominant.

Application

Transforming Growth Factor-β1 from porcine platelets has been used in chondrogenic differentiation. It has also been used in immobilization process.

Actions biochimiques/physiologiques

Transforming growth factor-β1 (TGF-β) is a multifunctional polypeptide growth factor. TGF-β mediates cell proliferation, differentiation and migration. Important cellular functions such as inflammation, extracellular matrix synthesis, epithelial restoration and developmental processes are also controlled by TGF-β. TGF-β controls the regulation of enamel mineralization in porcine. TGF-β is associated with the development of idiopathic pulmonary fibrosis (a chronic lung disease). TGF-β promotes epithelial-mesenchymal transition (EMT) and contributes to proliferation and metastasis of cancer. The TGF-β induced EMT is observed in kidney fibrosis. TGF-β is stimulates regeneration of intestinal tissue.

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin per μg cytokine.

Remarque sur l'analyse

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)