H8541

Facteur de croissance transformant β1 human

TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

• Synonyme(s) : TGF-β1
• Numéro MDL: MFCD00166089
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 300
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: ≤0.5 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: dimer 25 kDa (non-glycosylated)
• Conditionnement: pkg of 5 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P01137
• Température de stockage: -10 to -25°C
• Informations sur le gène: human ... TGFB1(7040)

Description

Description générale

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide. TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.

Application

Transforming Growth Factor-β1 (TGF-β) human has been used :

• to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.
• to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.
• to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.

Actions biochimiques/physiologiques

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Le facteur de croissance transformant β1 (TGF-β1) est produit par de nombreux types de cellules, mais d'après la littérature, c'est dans les plaquettes de mammifères que sa concentration est la plus élevée : elle y est environ quatre fois plus importante que la concentration en TGF-β2.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Remarque sur l'analyse

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H314
• Conseils de prudence: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Classification des risques: Eye Dam. 1 - Skin Corr. 1B
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable