The structure of the material after purification is not assessed. While the native folding state cannot be guaranteed, the final purified flagellan can bind human and mouse TLR5 and activate TLR5 in cell culture.
SRP8029
Flagellin from Salmonella typhimurium
>95% (SDS-PAGE)
Synonyme(s) :
FliC, Intelectin-1
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10 μG
273.00 CHF
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10 μG
273.00 CHF
About This Item
Produits recommandés
Source biologique
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Essai
>95% (SDS-PAGE)
Forme
lyophilized
Poids mol.
~50 kDa by SDS-PAGE
Conditionnement
pkg of 10 μg
Impuretés
<0.01 EU/μg endotoxin, tested
Couleur
white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ... fliC (STM1959)
Description générale
FliC (flagellin) is the subunit protein which polymerizes to form the filaments of bacterial flagella. The protein has four-domains (D0, D1, D2, D3).[1]
The encoded flagellin filament contains 55 kDa monomers.[2]
Actions biochimiques/physiologiques
FliC (flagellin) can activate the innate immune system by TLR5 (toll like receptor 5) and is an important proinflammatory molecule.[1][3] FliC also induces the production of antibodies and is identified as an antigen by CD4+ T cells.[4]
It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α.[5]
Forme physique
Lyophilized from PBS.
Reconstitution
Reconstitute with 100 μL deionized water.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Mucosal administration of flagellin induces innate immunity in the mouse lung.
Honko A N and Mizel S B
Infection and Immunity, 72(11), 6676-6679 (2004)
FliC-specific CD4+ T cell responses are restricted by bacterial regulation of antigen expression.
Cummings LA
Journal of Immunology, 174, 7929-7938 (2005)
Laís Rossi Perazza et al.
The Journal of nutrition, 150(10), 2673-2686 (2020-09-05)
Recent meta-analyses suggest that the consumption of fermented dairy products reduces type 2 diabetes and cardiovascular disease (CVD) risk, although the underlying mechanisms remain unclear. We evaluated whether dairy protein products modulated gut microbiota and cardiometabolic features in mouse models
In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted.
Cummings LA
Molecular Microbiology, 61, 795-809 (2006)
Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways.
Zeng H, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(1), G96-G108 (2006)
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Hello! I wondered if this product is expressed from the host in its native folded state? I am interested in obtaining native folded flagellin... thanks for any insights you can provide in this matter!
1 answer-
Helpful?
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Good morning, I would like to have the sequence of this protein and the host where it was produced. Thank yo and best regards
1 answer-
This is native Flagellin purified from Salmonella typhimurium. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P06179/entryHelpful?
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