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SHC005

Sigma-Aldrich

MISSION® eGFP shRNA Control Vector

shRNA sequence targeting eGFP

Synonyme(s) :

MISSION® Control Vectors

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About This Item

Numéro MDL:
Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

Gamme de produits

MISSION®

Concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

The MISSION® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761).[1] The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP.[2][3]
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids[4], MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Informations légales

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Myoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer.
Blomme A
Oncogene, 36(15), 2116-2130 (2017)
Arnaud Blomme et al.
Oncotarget, 7(50), 83669-83683 (2016-11-16)
Exosomes are communication mediators participating in the intercellular exchange of proteins, metabolites and nucleic acids. Recent studies have demonstrated that exosomes are characterized by a unique proteomic composition that is distinct from the cellular one. The mechanisms responsible for determining
Kanako Kono et al.
Developmental biology, 394(1), 142-155 (2014-07-06)
Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo
Huizhi Wang et al.
Oncogenesis, 9(8), 76-76 (2020-08-28)
Methyl-CpG-binding protein 2 (MeCP2) has been characterized as an oncogene in several types of cancer. However, its precise role in pancreatic ductal adenocarcinoma (PDAC) remains unclear. Hence, this study aimed to evaluate the potential role of MeCP2 in pancreatic cancer
Jun Won Park et al.
Biochemical and biophysical research communications, 498(3), 566-572 (2018-03-11)
TXNIP is a potent tumor suppressor with reduced expression in various types of human cancer. The prognostic and predictive power of TXNIP has been recognized in human breast cancer. The aim of this study is to investigate the clinical relevance

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