The particle size of the powder has not been measured.
S5761
Bicarbonate de sodium
Sodium bicarbonate
powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Synonyme(s) :
Hydrogénocarbonate de sodium
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500 G
71.80 CHF
1 KG
134.00 CHF
5 KG
431.00 CHF
Sélectionner une taille de conditionnement
Changer de vue
500 G
71.80 CHF
1 KG
134.00 CHF
5 KG
431.00 CHF
About This Item
Formule linéaire :
NaHCO3
Numéro CAS:
Poids moléculaire :
84.01
Beilstein:
4153970
Numéro CE :
Numéro MDL:
Code UNSPSC :
12161702
ID de substance PubChem :
Nomenclature NACRES :
NA.75
Essai:
99.5-100.5%
Forme:
powder
Solubilité:
water: soluble 100 mg/mL
Produits recommandés
Qualité
for molecular biology
300
Niveau de qualité
Gamme de produits
BioReagent
Essai
99.5-100.5%
Forme
powder
Technique(s)
cell culture | insect: suitable
cell culture | mammalian: suitable
pH
6.8-7.4
pKa (25 °C)
(1) 6.37, (2) 10.25 (carbonic acid)
Solubilité
water: soluble 100 mg/mL
Densité
2.16 g/mL at 25 °C (lit.)
Chaîne SMILES
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
Clé InChI
UIIMBOGNXHQVGW-UHFFFAOYSA-M
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Description générale
Le bicarbonate de sodium est couramment utilisé comme tampon physiologique dans les applications de culture cellulaire. Le pKa de la réaction bicarbonate <-> acide carbonique est de 6,4. Le pKa de la réaction bicarbonate <-> carbonate est de 10,3. Le bicarbonate de sodium présente deux pKa, à savoir 6,4 et 10,3. Il est couramment utilisé comme tampon physiologique dans les applications in vitro.
Application
Le bicarbonate de sodium a été utilisé :
- comme constituant du système de culture embryonnaire de bovin lors de la maturation, la fécondation et la culture in vitro.[1]
- comme constituant du mélange 1:1 de milieux DMEM (Dulbecco′s modified Eagle′s medium) et F12 de Ham pour la culture de cellules endothéliales microvasculaires bovines[2]
- comme constituant du milieu ITS (insuline, transferrine et sélénite) utilisé pour l′isolement de neurones à dopamine des cellules souches embryonnaires humaines[3]
- comme constituant du milieu de culture des cellules rénales embryonnaires humaines[4]
- comme constituant de la solution de décongélation de Beltsville utilisée pour la dilution du sperme[5]
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Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
L Keskintepe et al.
Biology of reproduction, 55(2), 333-339 (1996-08-01)
The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199
E Meyer et al.
The European journal of neuroscience, 11(3), 1105-1108 (1999-04-02)
The human slow poke (hSlo) K+ channel was tagged with GFP (green fluorescent protein) at the N-terminus of its alpha-subunit. The fusion protein was expressed transiently in HEK293 cells; it formed functional voltage-gated channels as shown by whole cell patch-clamp
M Solís et al.
Research in veterinary science, 83(3), 403-409 (2007-03-21)
Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x
K Okuda et al.
Biology of reproduction, 61(4), 1017-1022 (1999-09-24)
There is sufficient evidence to prove that tumor necrosis factor alpha (TNFalpha) modulates bovine corpus luteum (CL) function. Our previous study demonstrated that functional TNFalpha receptors are present on luteal cells in bovine CL throughout the estrous cycle. The purpose
Chang-Hwan Park et al.
Methods in molecular biology (Clifton, N.J.), 407, 311-322 (2008-05-06)
In this chapter, we introduce a co-culture protocol for human embryonic stem (hES) cell differentiation in which dopamine (DA) neurons with midbrain-specific markers are efficiently derived. Human ES cells on a feeder layer of stromal cells are induced to differentiate
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Nonylphenols (NP) are ubiquitous substances that have been detected in highly diverse foodstuff. As breakdown products of nonylphenol ethoxylates, NP could end up in the aquatic environment. This article presents a rapid method for NP detection in surface water by planar solid phase extraction – planar yeast estrogen screen (pSPE-pYES).
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What is the shelf life after delivery and opening? Is there usually an impact on quality after 2 years? The color of the medium changed and the pH decreased. Does the solubility or function of sodium bicarbonate decrease as it gets older?
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
After opening, various factors may influence the performance of the product. Moisture uptake and cross-contamination are common issues that are dependent on product handling and storage. If the product you are using is experience stability issues before the recommended restest date, this reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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