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RAB1156

Sigma-Aldrich

Rat TIMP-2 ELISA Kit

for cell culture supernatants, plasma, and serum samples

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

rat

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable

Entrée

sample type cell culture supernatant(s)
sample type serum sample(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/ml

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

rat ... Timp2(29543)

Description générale

Tissue inhibitor of metalloproteinase 2 (TIMP -2) is expressed in the brain especially in spinal motor neurons, cerebellar Purkinje cells, cerebral cortical, and hippocampal pyramidal cells. It is an inhibitor of matrix metalloproteinase and tumor angiogenesis. TIMP-2 is involved in matrix remodeling and stimulates quiescent cell proliferation. This ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine, and/or cell and tissue lysates.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.
Rat TIMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify tissue inhibitor of metalloproteinase 2 (TIMP -2) in U87MG human glioblastoma cells.

Autres remarques

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Diane M Jaworski et al.
Journal of neurochemistry, 98(1), 234-247 (2006-06-30)
Neuronal differentiation requires exquisitely timed cell cycle arrest for progenitors to acquire an appropriate neuronal cell fate and is achieved by communication between soluble signals, such as growth factors and extracellular matrix molecules. Here we report that the expression of
Dong-Wan Seo et al.
Cell, 114(2), 171-180 (2003-07-31)
Tissue inhibitors of metalloproteinases (TIMPs) suppress matrix metalloproteinase (MMP) activity critical for extracellular matrix turnover associated with both physiologic and pathologic tissue remodeling. We demonstrate here that TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo
Dimitra Bourboulia et al.
Oncotarget, 4(1), 166-176 (2013-02-02)
Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) plays an essential role in regulating matrix remodeling, cell growth, differentiation, angiogenesis and apoptosis in vitro and in vivo. We have recently shown that TIMP-2-mediated inhibition of tumor growth is independent of matrix metalloproteinase-mediated
Shan Wu et al.
International journal of molecular medicine, 42(6), 3386-3394 (2018-10-03)
The present study investigated the underlying molecular mechanism by which Buthus martensii Karsch chlorotoxin (BmK CT) inhibits the invasion and metastasis of glioma cells and the possibility of 131I‑labeled BmK CT (131I‑BmK CT) as a novel targeted agent for the treatment of glioma. The

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