P9041
Phosphodiesterase II from bovine spleen
lyophilized powder, ≥5.0 units/mg protein
Synonyme(s) :
3′-Exonuclease, Spleen exonuclease, Spleen phosphodiesterase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
10 UNITS
216.00 CHF
25 UNITS
760.00 CHF
Sélectionner une taille de conditionnement
Changer de vue
10 UNITS
216.00 CHF
25 UNITS
760.00 CHF
About This Item
Produits recommandés
Forme
lyophilized powder
Activité spécifique
≥5.0 units/mg protein
Poids mol.
65 kDa
Numéro d'accès UniProt
Activité étrangère
5′-Nucleotidase ≤1% of base activity
Température de stockage
−20°C
Informations sur le gène
cow ... PDE2A(281971)
Description générale
Research area: Cell signaling. Phosphodiesterase (PDE) II belongs to the PDE superfamily and includes subtypes from one to 11.[1] They exist as a dimer and comprise of N-terminal regulatory domain, C-terminal prenylated domain, and a Zn2+containing catalytic domain.[1] The bovine spleen PDE2 corresponds to a molecular weight of 65 kDa. It requires divalent cations for its enzymatic activity and has an optimum pH of 7.5.[2]
Application
Phosphodiesterase (PDE) is any enzyme that is used to break phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex[3]. Bovine spleen phosphodiesterase has been used to digest N-cadherin[4]. Phosphodiesterase II from bovine is suitable for sequential and end-group analysis of poly and oligonucleotides. It may be used in kinetic studieson substrate degradation of macro-molecules.
Phosphodiesterase II from bovine spleen has been used in the:
- excision of pyridyloxobutyl (POB) base adducts from DNA[5]
- digestion of DNA prior to cycloadenosine enrichment[6]
- hydrolysis of DNA from blue mussels gill tissue into deoxyribonucleoside 3′-monophosphates[7]
The product has been used in the characterization of polynucleotide chain length, base composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) base adducts from DNA.[5] Furthermore, it has been used along with micrococcal endonuclease to hydrolyze purified DNA to 3 -nucleoside monophosphates.[8]
Actions biochimiques/physiologiques
Hydrolyzes RNA, RNA-Core, 3′-alkyl- and 3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.
Phosphodiesterase (PDE) breaks phosphodiester bonds.[9] 3-Isobutyl-methylxanthine (IBMX) is a potential PDE2 inhibitor.[10]
The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme.
Définition de l'unité
One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37 °C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.
Remarque sur l'analyse
Protein determined by biuret
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Dynamic regulation of cAMP signaling by cGMP in the cardiovascular system: roles of phosphodiesterase 2 and phosphodiesterase 3 enzymes.
Donald H Maurice
Proceedings of the Western Pharmacology Society, 46, 32-36 (2004-01-01)
Hong Feng et al.
Biochemistry, 44(34), 11486-11495 (2005-08-24)
Endonuclease V is an enzyme that initiates a conserved DNA repair pathway by making an endonucleolytic incision at the 3' side one nucleotide from a deaminated base lesion. Site-directed mutagenesis analysis was conducted at seven conserved motifs of the thermostable
D H Bamford et al.
Journal of virology, 50(2), 309-315 (1984-05-01)
DNA of bacteriophage PRD1 has protein P8 at its termini. Extracts of infected cells are able to derivatize P8 in vitro with labeled dGTP. Two early proteins, P1 and P8, products of genes I and VIII, respectively, are the only
Characterization of novel phosphodiesterases in the bovine ovarian follicle
Sasseville M, et al.
Biology of Reproduction, 81(2), 415-425 (2009)
Ekaterina M Fock et al.
Pflugers Archiv : European journal of physiology, 448(2), 197-203 (2004-01-15)
The present study addressed the question of whether nitric oxide (NO) participates in regulation of osmotic water permeability in the urinary bladder of the frog Rana temporaria L. Experiments were carried out on isolated, paired hemi-bladders filled with amphibian Ringer
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
