This product is tested at pH 4.0 at 25C. The optimum pH and temperature have not been determined. However, the publication available at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153499/#:~:text=The%20pectinase%20has%20an%20estimated,temperature%20of%2061%C2%B0C.
P4716
Pectinase from Aspergillus niger
BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
Synonyme(s) :
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
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Date d'expédition estimée le30 mai 2025
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5000 UNITS
67.00 CHF
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123.00 CHF
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204.00 CHF
100000 UNITS
609.00 CHF
About This Item
67.00 CHF
Date d'expédition estimée le30 mai 2025
Produits recommandés
Source biologique
Aspergillus niger
Gamme de produits
BioReagent
Forme
aqueous glycerol solution
Activité spécifique
≥5 units/mg protein (Lowry)
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Technique(s)
cell culture | plant: suitable
Autre catégorie plus écologique
, Enabling
Température de stockage
2-8°C
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Description générale
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Application
Petctinase from Aspergillus niger has been used:
- for protoplasts liberation [1]
- in the protoplast enzyme mixture for enzyme digestion [2]
- to conduct partial saccharification of sugars
- to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Actions biochimiques/physiologiques
Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.
Définition de l'unité
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
Forme physique
Solution in 40% glycerol
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Protocoles
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
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Hello! Is 25 degrees Celsius and pH 4.0 the optimal temperature and pH for the highest activity of this enzyme? or do I still need to check? Thanks!
1 answer-
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