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P4306

Sigma-Aldrich

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

PDGF-BB

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About This Item

Numéro MDL:
MFCD00213023
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

1.0-3.0 ng/mL EC50

Qualité

endotoxin tested

Poids mol.

24.3 kDa

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/μg

Numéro d'accès UniProt

P01127

Température de stockage

−20°C

Informations sur le gène

human ... PDGFB(5155)

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Description générale

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Application

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

Actions biochimiques/physiologiques

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

Autres remarques

Dimer of the B chain of human PDGF.

Forme physique

Lyophilized from a 0.2 μm filtered solution.

Remarque sur l'analyse

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Overexpression of platelet-derived growth factor-BB increases tumor pericyte content via stromal-derived factor-1a/CXCR4 axis
Song N, et al.
Cancer Research, 69.15, 6057-6064 (2009)
Growth factors in the treatment of early osteoarthritis
Civinini R, et al.
Clinical Cases in Mineral and Bone Metabolism : The Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 10.1, 26-26 (2013)
A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair.
Schmidt MB, et al.
Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society, 14.5, 403-412 (2006)
The platelet derived growth factor-B polymorphism is associated with risk of severe fever with thrombocytopenia syndrome in Chinese individuals
Zhang XA, et al.
Oncotarget, 7(22), 33340-33349 (2016)
NIH 3T3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase C from Bacillus cereus acquire a transformed phenotype.
Johansen T, et al.
Molecular and Cellular Biology, 14.1, 646-654 (1994)
Afficher tous les articles scientifiques apparentés

Articles

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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