This product is also soluble in water at 10mg/ml. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/195/258/p4020pis.pdf
P4020
Protamine sulfate
amorphous powder
Synonyme(s) :
Salmine, Salmine from salmon
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1 G
54.30 CHF
5 G
93.80 CHF
10 G
233.00 CHF
25 G
432.00 CHF
100 G
1 440.00 CHF
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Changer de vue
1 G
54.30 CHF
5 G
93.80 CHF
10 G
233.00 CHF
25 G
432.00 CHF
100 G
1 440.00 CHF
About This Item
Produits recommandés
Nom du produit
Protamine sulfate salt from salmon, Grade X, amorphous powder
Type
Grade X
Niveau de qualité
Forme
amorphous powder
Impuretés
histone, free (Millon test)
Couleur
white to off-white
Solubilité
6 M HCl: 25 mg/mL
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Description générale
Application
Protamine sulfate salt from salmon has been used:
- in the pretreament of cells to study its effect on respiratory syncytial virus infection[4]
- in lentivirus production[5]
- to derivatize it into a diazeniumdiolate NO donor[6]
May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Actions biochimiques/physiologiques
Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
The design and evaluation of heparin-binding foldamers.
Sungwook Choi et al.
Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
Wenjie Ouyang et al.
Human gene therapy, 32(9-10), 481-494 (2020-12-02)
β-Thalassemia is one of the most prevalent genetic diseases worldwide. The current treatment for β-thalassemia is allogeneic hematopoietic stem cell transplantation, which is limited due to lack of matched donors. Gene therapy has been developed as an alternative therapeutic option
Steve N Bailey et al.
Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin
Diazeniumdiolation of protamine sulfate reverses mitogenic effects on smooth muscle cells and fibroblasts
Van Lith R, et al.
Free radical biology & medicine, 82, 13-21 (2015)
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Are there other solvents besides 6M HCl to bring protamine sulfate salt into solution?
1 answer-
Helpful?
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Bonjour, Pourriez vous me confirmer qu'il s'agit bien d'un polypeptide et de combien d'unité de monomère est il formé ? Pourriez vous m'envoyer la représentation de la molécule ? merci
1 answer-
Unfortunately, structural studies on for this product have not been performed. However, regarding structure characterization and molecular representation of protamine from salmon, the following publication may be of interest for reference.
J Biol Chem. 1959 Sep:234:2314-6.
Physical and chemical properties of protamine from the sperm of salmon (Oncorhynchus tschawytscha). II. Anion binding characteristics
https://pubmed.ncbi.nlm.nih.gov/13807973/Helpful?
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