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P3803

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

for molecular biology

Synonyme(s) :

DNA extraction

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100 ML
190.00 CHF
400 ML
579.00 CHF

190.00 CHF

Date d'expédition estimée le20 mars 2025

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100 ML
190.00 CHF
400 ML
579.00 CHF

About This Item

Code UNSPSC :
12190000
Nomenclature NACRES :
NA.52
Qualité:
for molecular biology
Essai:
≥99.0%
Application(s):
agriculture

190.00 CHF

Date d'expédition estimée le20 mars 2025

Qualité

for molecular biology

Niveau de qualité

200

Essai

≥99.0%

Forme

liquid

pH

6.5-6.9(pH of phenolic phase)

Application(s)

agriculture

Température de stockage

2-8°C

Application

Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions.[1][2][3][4] For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.

Avertissement

If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.

Autres remarques

This product has a shelf life of two years.

Produits recommandés

For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Organes cibles

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
MKCX1196
MKCV7671
MKCV3474
MKCV3475
MKCT9743

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Bing Yao et al.
Nature communications, 8(1), 1122-1122 (2017-10-27)
Chemical modifications on DNA molecules, such as 5-methylcytosine and 5-hydroxymethylcytosine, play important roles in the mammalian brain. A novel DNA adenine modification, N(6)-methyladenine (6mA), has recently been found in mammalian cells. However, the presence and function(s) of 6mA in the
Ying Cheng et al.
Human molecular genetics, 27(17), 2955-2964 (2018-05-24)
Autism spectrum disorders (ASDs) include a group of syndromes characterized by impaired language, social and communication skills, in addition to restrictive behaviors or stereotypes. However, with a prevalence of 1.5% in developed countries and high comorbidity rates, no clear underlying
Stephan C A Wens et al.
Circulation. Cardiovascular genetics, 9(1), 6-13 (2016-01-21)
Elevated plasma cardiac troponin T (cTnT) levels in patients with neuromuscular disorders may erroneously lead to the diagnosis of acute myocardial infarction or myocardial injury. In 122 patients with Pompe disease, the relationship between cTnT, cardiac troponin I, creatine kinase
Bizunesh Abere et al.
mBio, 11(6) (2020-12-17)
Viral noncoding RNAs have acquired increasing prominence as important regulators of infection and mediators of pathogenesis. Circular RNAs (circRNAs) generated by backsplicing events have been identified in several oncogenic human DNA viruses. Here, we show that Merkel cell polyomavirus (MCV)
Section 1 Isolation of nucleic acids
Isabel Ferrera
Biofouling, 88-88 (2014)
Afficher tous les articles scientifiques apparentés

Questions

1–3 of 3 Questions  

  1. I received two bottles. One for the phenol and one for the buffer. Do I need to add the phenol to the buffer? or the buffer already contains the phenol?

    1 answer

    1. Yes, the equilibration buffer is to be added before use, according to the following instructions:
      Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol reagent.
      Mix gently and allow the phases to separate before use, typically 2–4 hours.
      This will adjust the pH of the phenol phase from pH 6.7±0.2 to 8.0±0.2.

      Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/490/315/p3803pis.pdf

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?

    1 answer

    1. In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.

      Helpful?

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