The concentration of this antibody is reported on a lot specific basis. The product specification range is 6 - 12 mg/mL. A brief review of the lot history shows a typical range of 8 - 10 mg/mL. Please see the link below to review a sample or lot specific certificate:
https://www.sigmaaldrich.com/product/sigma/p2921#product-documentation
P2921
Monoclonal Anti-Protein A antibody produced in mouse
clone SPA-27, ascites fluid
Synonyme(s) :
Monoclonal Anti-Protein A
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100 μL
478.00 CHF
0.2 ML
648.00 CHF
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Changer de vue
100 μL
478.00 CHF
0.2 ML
648.00 CHF
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Conjugué:
unconjugated
application:
ELISA (i)
ICC
WB
ICC
WB
Clone:
SPA-27, monoclonal
Technique(s):
immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable
indirect ELISA: 1:20,000
western blot: suitable
citations:
19
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
SPA-27, monoclonal
Contient
15 mM sodium azide
Technique(s)
immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain.
Spécificité
Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.
Immunogène
protein A from Cowan I strain of Staphylococcus aureus.
Application
Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses.
Actions biochimiques/physiologiques
Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers.
Forme physique
Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ohood A Radhi et al.
The Journal of biological chemistry, 294(39), 14289-14307 (2019-08-08)
Autophagy plays multiple roles in host cells challenged with extracellular pathogens. Here, we aimed to explore whether autophagy inhibition could prevent bacterial infections. We first confirmed widely distinct patterns of autophagy responses in host cells infected with Staphylococcus aureus, as
A simple and robust method for the complete dissociation of HIV-1 p24 and other antigens from immune complexes in serum and plasma samples
Steindl F, et al.
Journal of Immunological Methods, 217(1-2), 143-151 (1998)
Shan Huang et al.
Frontiers in molecular biosciences, 7, 581095-581095 (2021-01-12)
Hog1 is a mitogen-activated protein kinase in yeast that primarily regulates cellular responses to hyperosmolarity stress. In this study, we have examined the potential involvement of Hog1 in mediating cellular responses to DNA damaging agents. We find that treatment of
Sandra K Schuller et al.
eLife, 9 (2020-11-17)
The yeast THO complex is recruited to active genes and interacts with the RNA-dependent ATPase Sub2 to facilitate the formation of mature export-competent messenger ribonucleoprotein particles and to prevent the co-transcriptional formation of RNA:DNA-hybrid-containing structures. How THO-containing complexes function at
Fabien Bonneau et al.
Genes & development, 37(11-12), 505-517 (2023-07-03)
Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate with mRNA-binding proteins to form messenger ribonucleoprotein (mRNP) complexes.
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I would like to know the concentration (mg/mL) of the anti-proA antibody. Thanks!
1 answer-
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