P1291
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Antiperoxidase, Pap Antibody, Peroxidase Anti Peroxidase, Peroxidase Anti Peroxidase - Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit, PAP
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About This Item
Produits recommandés
Source biologique
rabbit
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Forme
buffered aqueous solution
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Spécificité
By immunoelectrophoresis (IEP), a single arc of precipitation is observed when the product (prior to the addition of BSA) is electrophoresed and then allowed to diffuse against anti-rabbit whole serum or anti-rabbit IgG. No reaction is observed against free horseradish peroxidase.
Application
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit has been used in:
- immunoblotting
- to detect the expression of TAP-tagged proteins
- immunoprecipitation
- immunohistochemical staining
Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit was used in immunoblotting of yeast whole cell extracts.
Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Sigma Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Actions biochimiques/physiologiques
Peroxidase:anti-peroxidase (PAP) immune complex is inexpensive immunoglobulin ligand. It is widely used for the detection of fragment crystallisable (Fc) receptor binding and fragment antigen binding (Fab) regions.
The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Stockage et stabilité
Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing or storage in "frost-free" freezers, is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Vladimir Yong-Gonzales et al.
PloS one, 7(12), e51540-e51540 (2013-01-04)
The Smc5-Smc6 complex in Saccharomyces cerevisiae is both essential for growth and important for coping with genotoxic stress. While it facilitates damage tolerance throughout the genome under genotoxin treatment, its function during unperturbed growth is mainly documented for repetitive DNA
Distribution and relative frequency of immunohistochemically detected endocrine cells in the stomach of New Zealand White rabbit (Oryctolagus cuniculus)
Turk S, et al.
Iranian journal of veterinary research, 20(1), 39-45 (2019)
Amparo Cuéllar Pérez et al.
PloS one, 9(1), e84891-e84891 (2014-01-15)
Jasmonate (JA) signalling is mediated by the JASMONATE-ZIM DOMAIN (JAZ) repressor proteins, which are degraded upon JA perception to release downstream responses. The ZIM protein domain is characteristic of the larger TIFY protein family. It is currently unknown if the
Gisela Pöll et al.
PloS one, 9(12), e114898-e114898 (2014-12-17)
Eukaryotic ribosome biogenesis is a multistep process involving more than 150 biogenesis factors, which interact transiently with pre-ribosomal particles to promote their maturation. Some of these auxiliary proteins have been isolated in complexes found separate from the ribosomal environment. Among
Åsa Segerstolpe et al.
Nucleic acids research, 41(2), 1178-1190 (2012-11-30)
Ribosomal subunit biogenesis in eukaryotes is a complex multistep process. Mrd1 is an essential and conserved small (40S) ribosomal subunit synthesis factor that is required for early cleavages in the 35S pre-ribosomal RNA (rRNA). Yeast Mrd1 contains five RNA-binding domains
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