P0996

PARP-1 human

recombinant, expressed in E. coli

• Synonyme(s) : NAD⁺ ADP-ribosyltransferase, Poly(ADP-ribose) Polymerase
• Numéro de classification (Commission des enzymes): 2.4.2.30 (BRENDA, IUBMB)
• Numéro MDL: MFCD01324157
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Forme: solution
• Numéro d'accès UniProt: P09874
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... PARP1(142)

Description

Description générale

Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis. [1]

Poly(ADP-ribose) polymerase 1 (PARP1) belongs to the PARP family which is highly conserved during evolution. PARP1 gene is localized at 1q42.12 in the human chromosome.[2]

Application

PARP-1 human has been used in the GST-pull down assay for immunoprecipitation of Glutathione S-transferase (GST) or GST- cyclic GMP- AMP synthase (cGAS) (GST-cGAS).[3] It has also been used in in vitro ribosylation assay.[4]

PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD⁺, specifically binds Zn²⁺ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. [5] It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments. [6]

Actions biochimiques/physiologiques

PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis. [7]

PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn²⁺ and DNA, and recognizes single-strand breaks in DNA. It is involved in base excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 expression and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive HIV-1 infection.

Poly(ADP-ribose) polymerase 1 (PARP1) is essential for diverse functions like DNA damage detection and repair, chromatin modification, cell differentiation, transcription and apoptotic cell death. It also plays a major role in spermatogenesis. Polymorphism in the gene PARP1 is associated with oligospermia and causes male infertility.[2]

Définition de l'unité

One unit will incorporate 10 femptomole of poly(ADP-ribose) from NAD into 5 μg of immobilized histone proteins in 30 minutes at 22 °C in a 96 well plate.

Forme physique

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1% Triton^™-X 100, 50 % glycerol, and 0.1 mg/ml BSA.

Informations légales

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable