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N7271

Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonyme(s) :

Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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.2 UNIT
1 210.00 CHF

1 210.00 CHF


Date d'expédition estimée le26 mai 2025



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.2 UNIT
1 210.00 CHF

About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

1 210.00 CHF


Date d'expédition estimée le26 mai 2025


Source biologique

Streptococcus pneumoniae

Niveau de qualité

Forme

buffered aqueous solution

Concentration

≥5 units/mL

Activité étrangère

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

Streptococcus pneumoniae R6 ... nanB(933504)

Actions biochimiques/physiologiques

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Conditionnement

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Définition de l'unité

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Forme physique

Solution in 50 mM sodium phosphate, pH 7.5

Notes préparatoires

Expressed in glycosidase-free hosts.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
Jin-Hyo Kim et al.
Science (New York, N.Y.), 340(6128), 71-75 (2013-02-23)
Influenza antiviral agents play important roles in modulating disease severity and in controlling pandemics while vaccines are prepared, but the development of resistance to agents like the commonly used neuraminidase inhibitor oseltamivir may limit their future utility. We report here
Charng-Sheng Tsai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(7), 2466-2471 (2013-01-30)
Alkyne-hinged 3-fluorosialyl fluoride (DFSA) containing an alkyne group was shown to be a mechanism-based target-specific irreversible inhibitor of sialidases. The ester-protected analog DFSA (PDFSA) is a membrane-permeable precursor of DFSA designed to be used in living cells, and it was

Articles

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Questions

1–5 of 5 Questions  
  1. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

      Helpful?

  2. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    1 answer
    1. The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

      Helpful?

  5. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

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