N2635
Nucleoside 5′-Diphosphate Kinase from bovine liver
buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)
Synonyme(s) :
NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
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100 UNITS
249.00 CHF
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896.00 CHF
About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
bovine liver
Forme
buffered aqueous glycerol solution
Activité spécifique
≥1,000 units/mg protein (biuret)
Conditions de stockage
(Tightly closed)
Technique(s)
activity assay: suitable
Activité étrangère
lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
Température de stockage
2-8°C
Catégories apparentées
Description générale
Application
Nucleoside 5′-Diphosphate Kinase has been used:
- nucleoside diphosphate kinase (NDPK) assays[3]
- coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)[4]
- non-metastatic protein 23 (NM23) growth stimulation assay[5]
- in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells [6]
- in a study to investigate protein synthesis in rabbit reticulocytes[7]
Actions biochimiques/physiologiques
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs).[1] NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.[8]
Définition de l'unité
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.
Anticorps
Enzyme
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Substrat
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Shaobai Huang et al.
Annals of botany, 96(4), 703-715 (2005-07-20)
Anoxia-tolerant plant tissues synthesize a number of proteins during anoxia, in addition to the 'classical anaerobic proteins' involved in glycolysis and fermentation. The present study used a model system of rice coleoptile tips to elucidate patterns of protein synthesis in
Krisztina Takács-Vellai et al.
Cellular and molecular life sciences : CMLS, 72(8), 1447-1462 (2014-12-30)
In textbooks of biochemistry, nucleoside diphosphate conversion to a triphosphate by nucleoside diphosphate 'kinases' (NDPKs, also named NME or NM23 proteins) merits a few lines of text. Yet this essential metabolic function, mediated by a multimeric phosphotransferase protein, has effects
R Roy et al.
Biochemical and biophysical research communications, 122(3), 1418-1425 (1984-08-16)
Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above
Harjeet Kaur et al.
The Biochemical journal, 393(Pt 1), 235-243 (2005-09-15)
In growth-factor-stimulated signal transduction, cell-surface receptors recruit PI3Ks (phosphoinositide 3-kinases) and Ras-specific GEFs (guanine nucleotide-exchange factors) to the plasma membrane, where they produce 3'-phosphorylated phosphoinositide lipids and Ras-GTP respectively. As a direct example of pathway networking, Ras-GTP also recruits and
Sanjeev Mahanta et al.
PloS one, 3(4), e2054-e2054 (2008-05-01)
The MUC1 protein is aberrantly expressed on many solid tumor cancers. In contrast to its apical clustering on healthy epithelial cells, it is uniformly distributed over cancer cells. However, a mechanistic link between aberrant expression and cancer has remained elusive.
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