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MP0025

Sigma-Aldrich

Venor™ GeM Mycoplasma Detection Kit, PCR-based

Synonyme(s) :

PCR mycoplasma detection kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Utilisation

 kit sufficient for 25 tests

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Technique(s)

PCR: suitable

Application(s)

detection
microbiology

Température de stockage

2-8°C

Catégories apparentées

Description générale

The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Application

The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.[1][2]

Composants

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Stockage et stabilité

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Informations légales

Venor is a trademark of Minerva Biolabs GmbH

Composants de kit seuls

Réf. du produit
Description
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
Anbarasu Kannan et al.
Scientific reports, 7, 46102-46102 (2017-04-07)
Human papilloma virus-16 (HPV-16) associated oropharyngeal cancer (HPVOPC) is increasing alarmingly in the United States. We performed whole genome sequencing of a 44 year old, male HPVOPC subject diagnosed with moderately differentiated tonsillar carcinoma. We identified new somatic mutation in
Anthony J Bainor et al.
Cancer research, 77(19), 5339-5348 (2017-08-16)
Distinguishing between indolent and aggressive prostate adenocarcinoma remains a priority to accurately identify patients who need therapeutic intervention. SIN3B has been implicated in the initiation of senescence
Afficher tous les articles scientifiques apparentés

Articles

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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Protocoles

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Questions

1–8 of 8 Questions  

  1. Can the cell culture supernatant be stored for a period of time before using the kit to determine if mycoplasma is present?

    1 answer

    1. The cell culture supernatant (sample) can be stored for several days at 4 °C before using the kit to determine if mycoplasma is present. For long-term storage, the samples can be stored at -20 °C, either in their native state or after heat inactivation.

      Helpful?

  2. Can I freeze the samples to collect several before using them?

    1 answer

    1. Samples should be cell supernatants when possible. Cell pellets are not recommended. The high concentration of DNA in the pellet is often responsible for the non-specific bands. If cell pellets are the only material available for testing, then centrifugation is necessary following sample boiling in order to remove all cell debris.

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  3. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    1 answer

    1. The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

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  4. Can a cell lysate be used in the VenorGeM® Mycoplasma Kit, Product MP0025, assay?

    1 answer

    1. A cell lysate can contain PCR inhibitors.  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. The sample required for VenorGeM® Mycoplasma Kit, Product MP0025, is a cell culture supernatant.  Is this from adherent or suspension cells?

    1 answer

    1. The mycoplasma assay kit can be run on supernatants from either adherent or suspension cells. The centrifugation step during sample preparation will remove any cellular debris to limit PCR inhibitors.

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  7. Product MP0025, VenorGeM® Mycoplasma Kit, does not contain TAQ.  What  DNA polymerase should be used?

    1 answer

    1. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that can be used include Taq, Tfl, and Tth. The kit has been optimized for use with Product No. D9307.

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  8. When using Product MP0025, VenorGeM® Mycoplasma Kit, there is inhibition in the sample (no internal control band).  Can the sample be diluted and the assay repeated?

    1 answer

    1. Sigma Aldrich does not recommend diluting the sample and re-assay as this can result in false negatives (sample diluted below the detection limits of the assay).  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

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