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MAK122

Sigma-Aldrich

Phospholipid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Phospholipid Quantification Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
food and beverages
pharmaceutical

Méthode de détection

colorimetric
fluorometric

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Application

Phospholipid assay kit has been used in biochemical analysis[1] and phospholipid quantitation.[2][3][4][5]

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the detection of phospholipids in biological samples

Principe

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334,H412

Conseils de prudence

P261 - P273 - P284 - P501

Classification des risques

Aquatic Chronic 3 - Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
122CE11A20
122CE11A20
122CE08A29
122CD11A30
122CE08A29

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Inhibiting mevalonate pathway enzymes increases stromal cell resilience to a cholesterol-dependent cytolysin.
Griffin S, et al.
Scientific Reports, 7(1), 17050-17050 (2017)
Vineela Parvathaneni et al.
Pharmaceutics, 12(3) (2020-03-04)
Non-small cell lung cancer (NSCLC) is a global disorder, treatment options for which remain limited with resistance development by cancer cells and off-target events being major roadblocks for current therapies. The discovery of new drug molecules remains time-consuming, expensive, and
Hypoxia-inducible factor-1 alpha as a therapeutic target for primary effusion lymphoma.
Shrestha P, et al.
PLoS Pathogens, 13(9), e1006628-e1006628 (2017)
Zhizhou Kuang et al.
Virulence, 11(1), 1090-1107 (2020-08-28)
The virulence behaviors of many Gram-negative bacterial pathogens are governed by quorum-sensing (QS), a hierarchical system of gene regulation that relies on population density by producing and detecting extracellular signaling molecules. Although extensively studied under in vitro conditions, adaptation of
Zein Shaban Ibrahim et al.
Molecular medicine reports, 13(4), 3017-3026 (2016-03-05)
The progression of diabetic nephropathy (DN) is accelerated by smoking. The current study investigated the ability of curcumin to protect the kidneys against damage from oxidative stress induced by diabetes mellitus (DM) and nicotine (NC). A total of 24 male
Afficher tous les articles scientifiques apparentés

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Is the 10 ml of Assay Buffer in MAK122 sufficient for 100 assays? Also, what amount of sample/buffer should be used?

    1 answer

    1. 1. The general recommendation for homogenization is a 1:10 solid to buffer ratio, for example, 20mg solid with 200 uL of Buffer. However, this ratio may not always be optimal. If there are concerns about having enough assay buffer for both homogenization and assays, the homogenization can be run with a different homogenization buffer, provided that it contains at least 0.5% Triton X-100. Please note that the amount of Assay Buffer needed per sample should be enough to yield 40 microliters of supernatant, unless you choose to use your own homogenization buffer with 0.5% Triton X-100.

      2. The kit is usually tested on tissue samples. However, it has not been tested on tissues themselves.

      3. For homogenizing samples, they can be run through 10-20 passes in a Dounce homogenizer on ice or by sonication, preferably performed in an ice-water bath. The degree of tissue lysis can be checked under a microscope. After homogenization, it is recommended to centrifuge the homogenate at 14,000 g for 10 minutes.

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  4. Why is cold assay buffer listed as a potential cause for the assay not working correctly? Can a 37 or 25 degree water bath be used to thaw the assay buffer? Can the assay buffer be stored at room temperature instead of at -20 degrees?

    1 answer

    1. The troubleshooting bulletin for MAK122 suggests that if the assay does not work, it could be due to the assay buffer not being at room temperature. This is because the enzyme is more active at room temperature than in colder conditions. Although stability data for the assay buffer at room temperature is not available, it is recommended to store it at -20 degrees or evaluate the suitability of room temperature storage. While the composition of the assay buffer is proprietary, it is expected to be stable at room temperature. Additionally, a 37 or 25 degree C water bath can be used to thaw the assay buffer, with a caution against excessive heating and a recommendation to remove it from the water bath once it reaches room temperature.

      Helpful?

  5. To prepare the 0.5% Triton X-100 solution to dilute my samples, should I dilute Triton x-100 in water, in the assay buffer, or something else?

    1 answer

    1. The 0.5% Triton X-100 solution to be used as the sample diluent may be prepared using water.

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