Accéder au contenu
Merck
Toutes les photos(4)

Documents

M7133

Sigma-Aldrich

4-Methylumbelliferyl sulfate potassium salt

sulfatase substrate

Synonyme(s) :

Potassium 4-methylumbelliferyl sulfate

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(4)

About This Item

Formule empirique (notation de Hill):
C10H7KO6S
Numéro CAS:
15220-11-8
Poids moléculaire :
294.32
Numéro Beilstein :
3803203
Numéro CE :
239-272-2
Numéro MDL:
MFCD00016970
Code UNSPSC :
12352204
ID de substance PubChem :
24897141
Nomenclature NACRES :
NA.32

Pureté

≥99% (HPLC)

Forme

powder

Solubilité

water: 5 mg/mL, clear, colorless

Fluorescence

λex 334 nm; λem 370 nm (pH 10.4)
λex 360 nm; λem 449 nm (Reaction products)

Température de stockage

−20°C

Chaîne SMILES 

[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12

InChI

1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1

Clé InChI

CSOCSPXOODWGLJ-UHFFFAOYSA-M

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Actions biochimiques/physiologiques

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Junichi Enokizono et al.
Drug metabolism and disposition: the biological fate of chemicals, 35(6), 922-928 (2007-03-14)
Breast cancer resistance protein (Bcrp/Abcg2) is a member of the ATP-binding cassette transporter family with the ability to transport a variety of sulfate conjugates. In the present study, the regional expression and activity of Bcrp and sulfotransferases (SULTs/Sults) were investigated
Anthony D Verderosa et al.
Scientific reports, 11(1), 1569-1569 (2021-01-17)
Antibiotics are failing fast, and the development pipeline remains alarmingly dry. New drug research and development is being urged by world health officials, with new antibacterials against multidrug-resistant Gram-negative pathogens as the highest priority. Antivirulence drugs, which inhibit bacterial pathogenicity
Lihua Wang-Eckhardt et al.
Cells, 10(12) (2021-12-25)
Sulfatide synthesis in the human renal cancer cell line SMKT-R3 was strongly inhibited in the presence of low µM concentrations of AG-205, a progesterone receptor membrane component 1 (PGRMC1) antagonist. This was also the case in Chinese hamster ovary (CHO)
P M Edelbroek et al.
Journal of chromatography, 530(2), 347-358 (1990-09-14)
The anticoagulant phenprocoumon is mainly metabolized in humans to hydroxylated metabolites and their glucuronides. A method is described for the determination of phenprocoumon, 4'-hydroxyphenprocoumon, 6-hydroxyphenprocoumon, 7-hydroxyphenprocoumon, and their glucuronide and sulphate conjugates in human urine. Reversed-phase high-performance liquid chromatography is
O P van Diggelen et al.
Journal of inherited metabolic disease, 12(3), 273-280 (1989-01-01)
Arylsulphatase C (ASC) activity in leukocytes and fibroblasts measured with 4-methylumbelliferylsulphate, is caused by at least two genetically different sulphatases. One of these is steroid sulphatase (STS). Depending on the substrate concentration, about 10-50% of the ASC activity in leukocytes
Afficher tous les articles scientifiques apparentés

Articles

Drug Conjugate Analysis using β-Glucuronidases

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique