Really good value for money and always arrives with a good shelf-life in terms of expiry date. Great for adding cytokines + hydrocortisone to to create your own endothelial cell growth medium - has kept my HUVECs consistently happy since I started using it in 2018. Also great to use when isolating human primary endothelial cells.
M4530
Medium 199
With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
1 of 1 reviewers received a sample product or took part in a promotion
Synonyme(s) :
M199 Medium, TCM 199
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A propos de cet article
UNSPSC Code:
12352207
NACRES:
NA.75
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Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- as a suspension media to maintain the primary neuronal cultures
- as a component of the collagen gel for ex vivo embryonic heart culture
- to maintain the ovarian tissue sections as part of the slow-freezing protocol
1 of 1
Cet article | |||
|---|---|---|---|
| description With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture | description Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture |
| form liquid | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
| components NaHCO3: yes | components sodium pyruvate: no | components L-glutamine: yes | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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