M3817
MegaCell™ RPMI-1640 Medium
without L-glutamine, liquid, sterile-filtered, suitable for cell culture
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Produits recommandés
Stérilité
sterile-filtered
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Composants
L-glutamine: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
Catégories apparentées
Description générale
MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.
Application
MegaCell™ RPMI-1640 Medium has been used:
- as a component of the TSC medium for culturing trophoblast stem cells (TSC)
- as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
- as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs
Reconstitution
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Informations légales
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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