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L4895

Sigma-Aldrich

Lectine de Triticum vulgaris (blé)

FITC conjugate, lyophilized powder

Synonyme(s) :

Agglutinine de germe de blé, WGA

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2 MG
122.00 CHF
5 MG
245.00 CHF
10 MG
494.00 CHF

122.00 CHF


Date d'expédition estimée le06 avril 2025



Sélectionner une taille de conditionnement

Changer de vue
2 MG
122.00 CHF
5 MG
245.00 CHF
10 MG
494.00 CHF

About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

122.00 CHF


Date d'expédition estimée le06 avril 2025


Conjugué

FITC conjugate

Niveau de qualité

Forme

lyophilized powder

Puissance

<50 μg/mL agglutination activity

Composition

Protein, ~50% Lowry

Ampleur du marquage

~2 mol FITC per mol protein

Température de stockage

2-8°C

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Description générale

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]

Application

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence[2]
  • to stereotaxically inject into mice for neuronal tracing[3]
  • in immunohistochemistry for the analysis of myocytes[4]

Actions biochimiques/physiologiques

La WGA n′est pas spécifique à un groupe sanguin, mais elle présente une affinité pour les résidus N-acétyl-β-D-glucosaminyl et les oligomères du type N-acétyl-β-D-glucosamine. La WGA ne contient aucun glucide lié à une protéine.
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.[1]

Conditionnement

Package size based on protein content.

Forme physique

Contains phosphate buffer salts

Notes préparatoires

Les conjugués sont préparés à partir d′une lectine purifiée par affinité.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Sarah Aileen Lewis et al.
PloS one, 13(8), e0201986-e0201986 (2018-08-14)
Tissues with high-energy demand including the heart are rich in the energy-producing organelles, mitochondria, and sensitive to mitochondrial dysfunction. While alterations in mitochondrial function are increasingly recognized in cardiovascular diseases, the molecular mechanisms through which changes in mitochondria lead to
Robert H Oakley et al.
Science signaling, 12(577) (2019-04-18)
Stress is increasingly associated with heart dysfunction and is linked to higher mortality rates in patients with cardiometabolic disease. Glucocorticoids are primary stress hormones that regulate homeostasis through two nuclear receptors, the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), both
Novel Chemical Suppressors of Long QT Syndrome Identified by an In Vivo Functional Screen
Peal DS, et al.
Circulation (2011)
Rui Guo et al.
Scientific reports, 7(1), 8703-8703 (2017-08-20)
Diabetes is a major risk factor for cardiovascular disease and the lysosomal cysteine protease cathepsin K plays a critical role in cardiac pathophysiology. To expand upon our previous findings, we tested the hypothesis that, knockout of cathepsin K protects against
Chronic effects of mild hyperglycaemia on left ventricle transcriptional profile and structural remodelling in the spontaneously type 2 diabetic Goto-Kakizaki rat
Dsouza A, et al.
Heart Failure Reviews, 19(1), 65-74 (2014)

Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

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Questions

1–2 of 2 Questions  
  1. Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?

    1 answer
    1. This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
      https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdf

      Helpful?

  2. Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?

    1 answer
    1. There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.

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