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L2012

Lipopolysaccharides from Salmonella enterica serotype enteritidis

Name: Lipopolysaccharides from <I>Salmonella enterica</I> serotype enteritidis
Brand: SIGMA

purified by gel-filtration chromatography

Synonyme(s) :

LPS

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A propos de cet article

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131520

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Propriétés

biological source

Salmonella enterica (Serotype enteritidis)

Quality Segment

200

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is extracted from Salmonella enteritidis and purified by gel filtration. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Cet article	L4774	L6143	L7895
Sigma-Aldrich L2012 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L4774 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L6143 Lipopolysaccharides from Salmonella enterica serotype typhimurium	Sigma-Aldrich L7895 Lipopolysaccharides from Salmonella typhosa
description purified by gel-filtration chromatography	description purified by ion-exchange chromatography	description BioXtra, suitable for cell culture, γ-irradiated	description γ-irradiated, BioXtra, suitable for cell culture
biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (serotype typhimurium)	biological source bacterial (Salmonella typhosa)
solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow	solubility -	solubility -	solubility water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <1% Protein	impurities <1% Protein, <1% RNA	impurities <1% Protein (Lowry)	impurities <1% Protein (Lowry)

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Classe de stockage

11 - Combustible Solids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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