Accéder au contenu
Merck
Toutes les photos(2)

Principaux documents

E0887

Sigma-Aldrich

Esterase from rabbit liver

lyophilized powder, ≥30 units/mg protein

Synonyme(s) :

Carboxyl esterase, Carboxylic-ester hydrolase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

500 UNITS
254.00 CHF
1000 UNITS
721.00 CHF

254.00 CHF


Date d'expédition estimée le17 avril 2025



Sélectionner une taille de conditionnement

Changer de vue
500 UNITS
254.00 CHF
1000 UNITS
721.00 CHF

About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

254.00 CHF


Date d'expédition estimée le17 avril 2025


Forme

lyophilized powder

Niveau de qualité

Activité spécifique

≥30 units/mg protein

Poids mol.

60 kDa

Composition

Protein, ≥85% biuret

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Esterase belongs to the C-family of carboxylesterases.[1] It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.[2]

Application

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).
Esterase from rabbit liver has been used:
  • in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors[1]
  • in the catalysis and kinetic profiling studies of peptidic compounds[3]
  • in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)[4]
  • in the hydrolysis of oleuropein aglycone (Ole-AG)[5]

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.[6]

Actions biochimiques/physiologiques

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.[7]
Esterase enzyme has broad substrate specificity.[3] Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides.[8] Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.[9]

Définition de l'unité

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Forme physique

Lyophilized powder containing Tris buffer salts

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hansjörg Schwertz et al.
Journal of cardiovascular pharmacology, 52(2), 151-160 (2008-08-02)
Animal data strongly support a role for inflammation in myocardial ischemia reperfusion injury. Attempts at cardioprotection by immunomodulation (such as with the specific C5 antibody pexelizumab) in humans have been disappointing. We hypothesized that a broader spectrum antiinflammatory agent might
Anna Sobeková et al.
Acta biologica Hungarica, 60(1), 45-54 (2009-04-22)
The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after
Z Qiao et al.
The Journal of international medical research, 37(4), 1029-1037 (2009-09-19)
Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) contributes to atherosclerotic plaque instability and subsequent sudden coronary death. Statins are associated with decreased stroke risk and may improve stability of atherosclerotic plaques. The present study investigated the effect of simvastatin on expression of Lp-PLA(2)
Sisi Zhang et al.
Current protocols, 2(7), e489-e489 (2022-07-21)
Polysorbates (PSs), including PS20 and PS80, are non-ionic surfactants widely used in the pharmaceutical industry to enhance drug solubility and stability. Despite their wide application, PSs are prone to degradation by either hydrolysis or oxidation in drug formulations during storage;
Shijin Zhang et al.
Langmuir : the ACS journal of surfaces and colloids, 36(22), 6261-6267 (2020-05-19)
To explore the potential of step-by-step assembly in the fabrication of biological materials, we designed and synthesized two peptide-based molecules for enzyme-instructed hierarchical assembly. Upon the treatment of alkaline phosphatase, one molecule undergoes enzyme-instructed self-assembly forming uniformed nanofibers. The other

Protocoles

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Questions

  1. Is it possible for the esterase from rabbit liver (E0887) to hydrolyze ethyl hexanoate into hexanoic acid?

    1 answer
    1. The product has been evaluated for the conversion of ethyl butyrate to butyric acid and ethanol. However, there has been no evaluation for the hydrolysis of ethyl hexanoate into hexanoic acid, and there are no known citations of this product for ethyl hexanoate hydrolysis. Due to the structural similarity between ethyl hexanoate and ethyl butyrate, both containing an ester bond, it's possible that this product could hydrolyze ethyl hexanoate, as esterases typically accommodate a wide range of ester substrates. However, the rate and efficiency of the hydrolysis may differ from the enzyme activity tested using ethyl butyrate as a substrate.

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique