DNB200
GenElute™ Stool DNA Isolation Kit
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About This Item
Code UNSPSC :
41105600
Nomenclature NACRES :
NA.52
Produits recommandés
Caractéristiques du produit alternatif plus écologique
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Autre catégorie plus écologique
Conditions d'expédition
ambient
Température de stockage
room temp
Description générale
Sigma′s GenElute™ Stool DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from fresh or frozen stool samples. The kit can also be used to isolate DNA from preserved stool samples. The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acid using the provided Bead Tubes and a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
- Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
- Eliminates PCR inhibitors including humic acid
- High quality DNA for sensitive downstream applications
Application
- PCR
- Southern Blot Analysis
- Sequencing
- Microarray Analysis
Caractéristiques et avantages
- Fast and easy processing - Rapid isolation of high-quality, ready-to-use DNA in 30 minutes.
- No organic extraction or alcohol precipitation - Phenol is not required for the isolation
- Universal procedure - Simultaneous isolation of both host DNA and microbial DNA
- High yields - Consistent, high yields of inhibitor-free DNA up to 50 kb plus
- Remove all inhibitors - Eliminates all PCR inhibitors including humic acid
Définition de l'unité
Preps
Stockage et stabilité
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Autres remarques
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Informations légales
GenElute is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Risques supp
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Certificats d'analyse (COA)
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Doina Georgescu et al.
International journal of general medicine, 15, 5513-5531 (2022-06-16)
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Qi-da He et al.
Evidence-based complementary and alternative medicine : eCAM, 2019, 6184205-6184205 (2020-01-18)
In Traditional Chinese Medicine (TCM), moxibustion had been used for thousands of years. Many clinical case reports and scientific studies had proved that moxibustion had a good effect in treating acute gastric ulcer (AGU). Some studies had shown that the
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