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D0818

Sigma-Aldrich

Decalcifying Solution-Lite

aqueous solution, bp 208 °F

Synonyme(s) :

universal decalcifying agent

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About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Forme

aqueous solution

Durée de conservation

5 yr

Couleur

light yellow

Point d'ébullition

208 °F

Solubilité

water: soluble

Application(s)

hematology
histology

Température de stockage

room temp

Description générale

Decalcifying Solution-Lite is designed to be a universal and effective decalcifying agent.

Application

Decalcifying Solution-Lite is intended to be used for the decalcification of routine, immunohistochemical, and bone marrow core specimens.
It works equally well with all types of samples, including calvarial bone samples in an easy-to-handle time frame.

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Farhana Begum et al.
Journal of neuroscience methods, 187(1), 59-66 (2010-01-02)
Histological analysis of bone encased tissue is severely hampered by technical difficulties associated with sectioning calcified tissue. Cryosectioning of bone is possible but requires significant technical adaptation and expensive materials and is often time-consuming. Some decalcifying reagents in common use
A Merchán-Pérez et al.
Histochemistry and cell biology, 112(2), 125-130 (1999-08-25)
An ascorbic acid decalcifying solution was applied to immuno- and affinohistochemical studies on the inner ear. Rat inner ears fixed in 4% paraformaldehyde in PBS or in 2% acetic acid in ethanol solutions were adequately decalcified in an ascorbic acid
P J Emans et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 80(3-4), 111-115 (2005-11-22)
Apoptosis is characterized by DNA strand breaks with a 3'-OH terminus, which are analyzed by terminal deoxy(d)-UTP nick end labeling (TUNEL). Proteinase K digestion is thought to be an essential step in the TUNEL procedure. The effects of decalcifying reagents
Roger W Byard et al.
Journal of forensic sciences, 55(5), 1356-1358 (2010-05-22)
To determine whether routine decalcification may reduce the amount of stainable iron that is visible on tissue sections, samples of liver and lung tissue with excessive iron stores were placed in three standard decalcifying solutions (i) formic acid [33%], formaldehyde
L F Machado-Silveiro et al.
International endodontic journal, 37(6), 365-369 (2004-06-10)
To measure the demineralization capability of 1 and 10% citric acid, 10% sodium citrate and 17% EDTA during immersions of 5, 10 and 15 min on root canal dentine. Crowns were sectioned from eight maxillary canines. The cementum was removed

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