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Principaux documents

CWA-1080

Sigma-Aldrich

Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, (Sold Separately))

Synonyme(s) :

Tumor necrosis factor ligand superfamily member 6

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

NOK-1, monoclonal

Gamme de produits

ColorWheel®

Forme

lyophilized

Poids mol.

calculated mol wt 31.49 kDa

Produit purifié par

using Protein A

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

flow cytometry: suitable

Isotype

IgG1κ

Séquence de l'épitope

C-terminal

Numéro d'accès Protein ID

Numéro d'accès UniProt

Compatibilité

for use with ColorWheel® Dyes (Required, (Sold Separately))

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... FASLG(356)

Description générale

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Spécificité

Clone NOK-1 is a mouse monoclonal antibody that detects CD178 (FasL).

Immunogène

L5178Y mouse T lymphoma cells expressing recombinant human CD178.

Application

Quality Control Testing

Evaluated by Flow Cytometry in HEK293 cells transfected with CD178.

Staining of one million transiently CD178-transfected HEK 293 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-ready Allophycocyanin (APC) Dye.

Evaluated by Flow Cytometry in NK cells.

Flow Cytometry Analysis: Staining of one million Human NK cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178 (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilité

Requires ColorWheel® Dye (Sold Separately)

Description de la cible

Tumor necrosis factor ligand superfamily member 6 (UniProt: P48023; also known as Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178) is encoded by the FASLG (also known as APT1LG1, CD95L, FASL, TNFSF6) gene (Gene ID: 356) in human. Fas Ligand is a single-pass, homotrimeric, type II membrane protein with a cytoplasmic domain (aa 1-80), a transmembrane domain (aa 81-102), and an extracellular domain (aa 103-281). The membrane-bound FasL (mFasL) undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10, which produces an N-terminal fragment that lacks the receptor-binding extracellular domain. This ADAM10-processed FasL remnant form is still membrane anchored and further processed by signal peptide peptidase like 2A (SPPL2A) that liberates the FasL intracellular domain (FasL ICD). mFasL can also undergo proteolytic cleavage to produce a truncated soluble form (sFasL; aa 130-281). However, sFasL does not activate Fas, and it competes with the membrane bound FasL to reduce its cytotoxic activity. sFasL can bind to Fas, but does not induce its oligomerization, thereby preventing activation of the proapoptotic signaling pathway by the membrane bound FasL. FasL can also undergo N-glycosylation that enhances its apoptotic activity. Two isoforms of FasL have been described that are produced by alternative splicing. (Ref.: Volpe, E., et al. (2016). Front. Immunol. 7; 382; Schneider, P., et al. (1997). J. Biol. Chem. 272(30); 18827-18833).

Forme physique

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Informations légales

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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