CRISPR21

Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)

• Code UNSPSC : 41106610
• Nomenclature NACRES : NA.51

Propriétés

• Forme: liquid
• Niveau de qualité: 200
• Conditionnement: vial of 50 μL
• Concentration: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• Application(s): CRISPR
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

The Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector) is a critical positive control to monitor transfection efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control is a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and hPGK-driven Puromycin and BFP expression. This control requires Cas9 be delivered by a separate vector.

Application

Functional Genomics/Screening/Target Validation

Autres remarques

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

Informations légales

CRISPR Use License Agreement

Lentiviral License Agreement

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable