CRISPR18

Lenti CRISPR Universal Non-Target Control#1 Plasmid DNA (LV04 vector)

• Code UNSPSC : 41106609
• Nomenclature NACRES : NA.51

Propriétés

• Forme: liquid
• Niveau de qualité: 200
• Conditionnement: vial of 50 μL
• Concentration: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• Application(s): CRISPR
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Application

Functional Genomics/Screening/Target Validation

Autres remarques

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

Informations légales

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable